3hoz

From Proteopedia

Jump to: navigation, search


3hoz, resolution 3.65Å ()
Ligands: ,
Non-Standard Residues:
Activity: DNA-directed RNA polymerase, with EC number 2.7.7.6
Related: 3hou, 3hov, 3how, 3hox, 3hoy
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Complete RNA polymerase II elongation complex IV with a T-U mismatch and a frayed RNA 3'-guanine

Publication Abstract from PubMed

We show that RNA polymerase (Pol) II prevents erroneous transcription in vitro with different strategies that depend on the type of DNARNA base mismatch. Certain mismatches are efficiently formed but impair RNA extension. Other mismatches allow for RNA extension but are inefficiently formed and efficiently proofread by RNA cleavage. X-ray analysis reveals that a TU mismatch impairs RNA extension by forming a wobble base pair at the Pol II active center that dissociates the catalytic metal ion and misaligns the RNA 3' end. The mismatch can also stabilize a paused state of Pol II with a frayed RNA 3' nucleotide. The frayed nucleotide binds in the Pol II pore either parallel or perpendicular to the DNA-RNA hybrid axis (fraying sites I and II, respectively) and overlaps the nucleoside triphosphate (NTP) site, explaining how it halts transcription during proofreading, before backtracking and RNA cleavage.

Structural basis of transcription: mismatch-specific fidelity mechanisms and paused RNA polymerase II with frayed RNA., Sydow JF, Brueckner F, Cheung AC, Damsma GE, Dengl S, Lehmann E, Vassylyev D, Cramer P, Mol Cell. 2009 Jun 26;34(6):710-21. PMID:19560423

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3hoz is a 15 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

See Also

Reference

  • Sydow JF, Brueckner F, Cheung AC, Damsma GE, Dengl S, Lehmann E, Vassylyev D, Cramer P. Structural basis of transcription: mismatch-specific fidelity mechanisms and paused RNA polymerase II with frayed RNA. Mol Cell. 2009 Jun 26;34(6):710-21. PMID:19560423 doi:10.1016/j.molcel.2009.06.002

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools