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3gb8
From Proteopedia
| 3gb8, resolution 2.90Å () | |||||
|---|---|---|---|---|---|
| Gene: | CRM1, XPO1 (Homo sapiens), RNUT1, SNUPN, SPN1 (Homo sapiens) | ||||
| |||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||
| Coordinates: | save as pdb, mmCIF, xml | ||||
Crystal structure of CRM1/Snurportin-1 complex
CRM1 (also known as XPO1 and exportin 1) mediates nuclear export of hundreds of proteins through the recognition of the leucine-rich nuclear export signal (LR-NES). Here we present the 2.9 A structure of CRM1 bound to snurportin 1 (SNUPN). Snurportin 1 binds CRM1 in a bipartite manner by means of an amino-terminal LR-NES and its nucleotide-binding domain. The LR-NES is a combined alpha-helical-extended structure that occupies a hydrophobic groove between two CRM1 outer helices. The LR-NES interface explains the consensus hydrophobic pattern, preference for intervening electronegative residues and inhibition by leptomycin B. The second nuclear export signal epitope is a basic surface on the snurportin 1 nucleotide-binding domain, which binds an acidic patch on CRM1 adjacent to the LR-NES site. Multipartite recognition of individually weak nuclear export signal epitopes may be common to CRM1 substrates, enhancing CRM1 binding beyond the generally low affinity LR-NES. Similar energetic construction is also used in multipartite nuclear localization signals to provide broad substrate specificity and rapid evolution in nuclear transport.
Structural basis for leucine-rich nuclear export signal recognition by CRM1., Dong X, Biswas A, Suel KE, Jackson LK, Martinez R, Gu H, Chook YM, Nature. 2009 Apr 30;458(7242):1136-41. Epub 2009 Apr 1. PMID:19339969
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3GB8 is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Dong X, Biswas A, Suel KE, Jackson LK, Martinez R, Gu H, Chook YM. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature. 2009 Apr 30;458(7242):1136-41. Epub 2009 Apr 1. PMID:19339969 doi:10.1038/nature07975
Page seeded by OCA on Wed May 20 15:08:50 2009
