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3g33
From Proteopedia
| 3g33, resolution 3.00Å () | |||||||||
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| Gene: | CDK4 (Homo sapiens), CCND3, hCG_16683 (Homo sapiens) | ||||||||
| Activity: | Cyclin-dependent kinase, with EC number 2.7.11.22 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Crystal structure of CDK4/cyclin D3
Cyclin-dependent kinase 4 (CDK4)/cyclin D complexes are expressed early in the G(1) phase of the cell cycle and stimulate the expression of genes required for G(1) progression by phosphorylation of the product of the retinoblastoma gene, pRb. To elaborate the molecular pathway of CDK4 activation and substrate selection we have determined the structure of nonphosphorylated CDK4/cyclin D3. This structure of an authentic CDK/cyclin complex shows that cyclin binding may not be sufficient to drive the CDK active site toward an active conformation. Phosphorylated CDK4/cyclin D3 is active as a pRb kinase and is susceptible to inhibition by p27(Kip1). Unlike CDK2/cyclin A, CDK4/cyclin D3 can be inactivated by treatment with lambda-phosphatase, implying that phosphorylated T172 is accessible to a generic phosphatase while bound to a cyclin. Taken together, these results suggest that the structural mechanism of CDK4/cyclin D3 activation differs markedly from that of previously studied CDK/cyclin complexes.
The structure of CDK4/cyclin D3 has implications for models of CDK activation., Takaki T, Echalier A, Brown NR, Hunt T, Endicott JA, Noble ME, Proc Natl Acad Sci U S A. 2009 Feb 23. PMID:19237555
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
Disease
Known disease associated with this structure: Melanoma OMIM:[123829]
About this Structure
3G33 is a 4 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Takaki T, Echalier A, Brown NR, Hunt T, Endicott JA, Noble ME. The structure of CDK4/cyclin D3 has implications for models of CDK activation. Proc Natl Acad Sci U S A. 2009 Feb 23. PMID:19237555
Page seeded by OCA on Thu Apr 2 16:23:43 2009
Categories: Cyclin-dependent kinase | Homo sapiens | Brown, N R. | Echalier, A. | Endicott, J A. | Hunt, T. | Noble, M E.M. | Takaki, T. | Atp-binding | Cell cycle | Cell division | Cyclin | Disease mutation | Kinase | Nucleotide-binding | Phosphoprotein | Phosphorylation | Polymorphism | Proto-oncogene | Ser/thr protein kinase | Serine/threonine-protein kinase | Transferase
