3g05
From Proteopedia
Crystal structure of N-terminal domain (2-550) of E.coli MnmG
Structural highlights
FunctionMNMG_ECO57 NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe MnmE-MnmG complex is involved in tRNA modification. We have determined the crystal structure of Escherichia coli MnmG at 2.4-A resolution, mutated highly conserved residues with putative roles in flavin adenine dinucleotide (FAD) or tRNA binding and MnmE interaction, and analyzed the effects of these mutations in vivo and in vitro. Limited trypsinolysis of MnmG suggests significant conformational changes upon FAD binding. Structure-function analysis of Escherichia coli MnmG (GidA), a highly conserved tRNA-modifying enzyme.,Shi R, Villarroya M, Ruiz-Partida R, Li Y, Proteau A, Prado S, Moukadiri I, Benitez-Paez A, Lomas R, Wagner J, Matte A, Velazquez-Campoy A, Armengod ME, Cygler M J Bacteriol. 2009 Dec;191(24):7614-9. Epub 2009 Oct 2. PMID:19801413[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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