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3ewe

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3ewe, resolution 3.50Å ()
Gene: SEH1, YGL100W (Saccharomyces cerevisiae), J1624, NUP85, RAT9, YJR042W (Saccharomyces cerevisiae)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Crystal Structure of the Nup85/Seh1 Complex

Publication Abstract from PubMed

Nuclear pore complexes (NPCs) facilitate nucleocytoplasmic transport. These massive assemblies comprise an eight-fold symmetric scaffold of architectural proteins and central-channel phenylalanine-glycine-repeat proteins forming the transport barrier. We determined the Nup85*Seh1 structure, a module in the heptameric Nup84 complex, at 3.5 A resolution. Structural, biochemical, and genetic analyses position the Nup84 complex in two peripheral NPC rings. We establish a conserved tripartite element, the ancestral coatomer element ACE1, that reoccurs in several nucleoporins and vesicle coat proteins, providing structural evidence of coevolution from a common ancestor. We identify interactions that define the organization of the Nup84 complex based on comparison with vesicle coats and confirmed the sites by mutagenesis. We propose the NPC scaffold, like vesicle coats, is composed of polygons with vertices and edges forming a membrane-proximal lattice providing docking sites for additional nucleoporins.

Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats., Brohawn SG, Leksa NC, Spear ED, Rajashankar KR, Schwartz TU, Science. 2008 Oct 30. PMID:18974315

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3ewe is a 4 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

See Also

Reference

  • Brohawn SG, Leksa NC, Spear ED, Rajashankar KR, Schwartz TU. Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats. Science. 2008 Oct 30. PMID:18974315

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