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3dkt
From Proteopedia
| 3dkt, resolution 3.10Å () | |||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Crystal structure of Thermotoga maritima encapsulin
Compartmentalization is an important organizational feature of life. It occurs at varying levels of complexity ranging from eukaryotic organelles and the bacterial microcompartments, to the molecular reaction chambers formed by enzyme assemblies. The structural basis of enzyme encapsulation in molecular compartments is poorly understood. Here we show, using X-ray crystallographic, biochemical and EM experiments, that a widespread family of conserved bacterial proteins, the linocin-like proteins, form large assemblies that function as a minimal compartment to package enzymes. We refer to this shell-forming protein as 'encapsulin'. The crystal structure of such a particle from Thermotoga maritima determined at 3.1-angstroms resolution reveals that 60 copies of the monomer assemble into a thin, icosahedral shell with a diameter of 240 angstroms. The interior of this nanocompartment is lined with conserved binding sites for short polypeptide tags present as C-terminal extensions of enzymes involved in oxidative-stress response.
Structural basis of enzyme encapsulation into a bacterial nanocompartment., Sutter M, Boehringer D, Gutmann S, Gunther S, Prangishvili D, Loessner MJ, Stetter KO, Weber-Ban E, Ban N, Nat Struct Mol Biol. 2008 Sep;15(9):939-47. PMID:19172747
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3dkt is a 20 chain structure with sequence from Thermotoga maritima. The June 2009 RCSB PDB Molecule of the Month feature on Vaults by David Goodsell is 10.2210/rcsb_pdb/mom_2009_6. Full crystallographic information is available from OCA.
See Also
Reference
- Sutter M, Boehringer D, Gutmann S, Gunther S, Prangishvili D, Loessner MJ, Stetter KO, Weber-Ban E, Ban N. Structural basis of enzyme encapsulation into a bacterial nanocompartment. Nat Struct Mol Biol. 2008 Sep;15(9):939-47. PMID:19172747
Categories: RCSB PDB Molecule of the Month | Thermotoga maritima | Vaults | Ban, N. | Boehringer, D. | Gutmann, S. | Sutter, M. | Weber-Ban, E. | Antibiotic | Antimicrobial | Bacteriocin | Cobalt | Enzyme encapsulation | Ferritin-like protein | Hk97-fold | Hydrolase | Nanocompartment | Oxidative stress | Protease | Secreted | Structural protein-virus like particle complex
