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3di2
From Proteopedia
| 3di2, resolution 2.70Å () | |
|---|---|
| Sites: | |
| Ligands: | |
| Gene: | IL7 (Homo sapiens), IL7R (Homo sapiens) |
| Related: | 3di3
|
| Resources: | FirstGlance, OCA, PDBsum, RCSB |
| Coordinates: | save as pdb, mmCIF, xml |
Contents |
Crystal structure of the complex of human interleukin-7 with unglycosylated human interleukin-7 receptor alpha ectodomain
IL-7 and IL-7Ralpha bind the gamma(c) receptor, forming a complex crucial to several signaling cascades leading to the development and homeostasis of T and B cells. We report that the IL-7Ralpha ectodomain uses glycosylation to modulate its binding constants to IL-7, unlike the other receptors in the gamma(c) family. IL-7 binds glycosylated IL-7Ralpha 300-fold more tightly than unglycosylated IL-7Ralpha, and the enhanced affinity is attributed primarily to an accelerated on rate. Structural comparison of IL-7 in complex to both forms of IL-7Ralpha reveals that glycosylation does not participate directly in the binding interface. The SCID mutations of IL-7Ralpha locate outside the binding interface with IL-7, suggesting that the expressed mutations cause protein folding defects in IL-7Ralpha. The IL-7/IL-7Ralpha structures provide a window into the molecular recognition events of the IL-7 signaling cascade and provide sites to target for designing new therapeutics to treat IL-7-related diseases.
Structural and Biophysical Studies of the Human IL-7/IL-7Ralpha Complex., McElroy CA, Dohm JA, Walsh ST, Structure. 2009 Jan;17(1):54-65. PMID:19141282
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
Disease
Known disease associated with this structure: Severe combined immunodeficiency, T-cell negative, B-cell/natural killer cell-positive type OMIM:[146661], Multiple sclerosis, susceptibility to OMIM:[146661]
About this Structure
3DI2 is a 4 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- McElroy CA, Dohm JA, Walsh ST. Structural and biophysical studies of the human IL-7/IL-7Ralpha complex. Structure. 2009 Jan 14;17(1):54-65. PMID:19141282 doi:10.1016/j.str.2008.10.019
Page seeded by OCA on Wed Jan 28 09:45:38 2009
Categories: Homo sapiens | Dohm, J A. | McElroy, C A. | Walsh, S T.R. | Alternative splicing | Cytokine | Cytokine receptor | Cytokine/cytokine receptor complex | Disease mutation | Ectodomain | Glycoprotein | Growth factor | Interleukin | Membrane | Phosphoprotein | Polymorphism | Receptor | Scid | Secreted | Transmembrane
