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3df1
From Proteopedia
Contents |
Crystal structure of the bacterial ribosome from Escherichia coli in complex with hygromycin B. This file contains the 30S subunit of the first 70S ribosome, with hygromycin B bound. The entire crystal structure contains two 70S ribosomes.
Aminoglycosides are one of the most widely used and clinically important classes of antibiotics that target the ribosome. Hygromycin B is an atypical aminoglycoside antibiotic with unique structural and functional properties. Here we describe the structure of the intact Escherichia coli 70S ribosome in complex with hygromycin B. The antibiotic binds to the mRNA decoding center in the small (30S) ribosomal subunit of the 70S ribosome and induces a localized conformational change, in contrast to its effects observed in the structure of the isolated 30S ribosomal subunit in complex with the drug. The conformational change in the ribosome caused by hygromycin B binding differs from that induced by other aminoglycosides. Also, in contrast to other aminoglycosides, hygromycin B potently inhibits spontaneous reverse translocation of tRNAs and mRNA on the ribosome in vitro. These structural and biochemical results help to explain the unique mode of translation inhibition by hygromycin B.
Structural basis for hygromycin B inhibition of protein biosynthesis., Borovinskaya MA, Shoji S, Fredrick K, Cate JH, RNA. 2008 Aug;14(8):1590-9. Epub 2008 Jun 20. PMID:18567815
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3df1 is a 21 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein S10
- Ribosomal protein S11
- Ribosomal protein S12
- Ribosomal protein S13
- Ribosomal protein S14
- Ribosomal protein S15
- Ribosomal protein S16
- Ribosomal protein S17
- Ribosomal protein S18
- Ribosomal protein S19
- Ribosomal protein S2
- Ribosomal protein S20
- Ribosomal protein S21
- Ribosomal protein S3
- Ribosomal protein S4
- Ribosomal protein S5
- Ribosomal protein S6
- Ribosomal protein S7
- Ribosomal protein S8
- Ribosomal protein S9
- Ribosome
Reference
- Borovinskaya MA, Shoji S, Fredrick K, Cate JH. Structural basis for hygromycin B inhibition of protein biosynthesis. RNA. 2008 Aug;14(8):1590-9. Epub 2008 Jun 20. PMID:18567815 doi:10.1261/rna.1076908
