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3cx2
From Proteopedia
| 3cx2, resolution 1.30Å () | |||||||||
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| Gene: | MYBPC3 (Homo sapiens) | ||||||||
| Related: | 2v6h
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Crystal structure of the C1 domain of cardiac isoform of myosin binding protein-C at 1.3A
Myosin-binding protein C (MyBP-C) is a myofibril-associated protein found in cardiac and skeletal muscle. The cardiac isoform (cMyBP-C) is subject to reversible phosphorylation and the surface-charge state of the protein is of keen interest with regard to understanding the inter-protein interactions that are implicated in its function. Diffraction data from the C1 domain of cMyBP-C were extended to 1.30 A resolution, where the <I/sigma(I)> of the diffraction data crosses 2.0, using intense synchrotron radiation. The protonation-state determinations were not above 2sigma (the best was 1.81sigma) and therefore an extrapolation is given, based on 100% data completeness and the average DPI, that a 3sigma determination could be possible if X-ray data could be measured to 1.02 A resolution. This might be possible via improved crystallization or multiple sample evaluation, e.g. using robotics or a yet more intense/collimated X-ray beam or combinations thereof. An alternative would be neutron protein crystallography at 2 A resolution, where it is estimated that for the unit-cell volume of the cMyBP-C C1 domain crystal a crystal volume of 0.10 mm3 would be needed with fully deuterated protein on LADI III. These efforts would optimally be combined in a joint X-ray and neutron model refinement.
An investigation into the protonation states of the C1 domain of cardiac myosin-binding protein C., Fisher SJ, Helliwell JR, Khurshid S, Govada L, Redwood C, Squire JM, Chayen NE, Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):658-64. Epub 2008, May 14. PMID:18560154
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
Disease
Known disease associated with this structure: Cardiomyopathy, dilated, 115200 (3) OMIM:[600958], Cardiomyopathy, familial hypertrophic, 4 OMIM:[600958]
About this Structure
3CX2 is a 1 chain structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Fisher SJ, Helliwell JR, Khurshid S, Govada L, Redwood C, Squire JM, Chayen NE. An investigation into the protonation states of the C1 domain of cardiac myosin-binding protein C. Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):658-64. Epub 2008, May 14. PMID:18560154 doi:10.1107/S0907444908008792
Page seeded by OCA on Tue Feb 17 22:16:09 2009
Categories: Homo sapiens | Chayen, N E. | Fisher, S J. | Govada, L. | Helliwell, J R. | Khurshid, S. | Redwood, C. | Squire, J M. | Actin-binding | Cardiomyopathy | Cell adhesion | Contractile protein | Disease mutation | Immunoglobulin domain | Muscle protein | Myosin-binding protein | Phosphoprotein | Polymorphism | Protonation state | Thick filament
