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3ctr
From Proteopedia
| 3ctr, resolution 2.10Å () | |||||||||
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| Sites: | |||||||||
| Ligands: | |||||||||
| Gene: | PARN, DAN (Homo sapiens) | ||||||||
| Activity: | Poly(A)-specific ribonuclease, with EC number 3.1.13.4 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of the RRM-domain of the poly(A)-specific ribonuclease PARN bound to m7GTP
Poly(A)-specific ribonuclease (PARN) is a processive 3'-exoribonuclease involved in the decay of eukaryotic mRNAs. Interestingly, PARN interacts not only with the 3' end of the mRNA but also with its 5' end as PARN contains an RRM domain that specifically binds both the poly(A) tail and the 7-methylguanosine (m(7)G) cap. The interaction of PARN with the 5' cap of mRNAs stimulates the deadenylation activity and enhances the processivity of this reaction. We have determined the crystal structure of the PARN-RRM domain with a bound m(7)G triphosphate nucleotide, revealing a novel binding mode for the m(7)G cap. The structure of the m(7)G binding pocket is located outside of the canonical RNA-binding surface of the RRM domain and differs significantly from that of other m(7)G-cap-binding proteins. The crystal structure also shows a remarkable conformational flexibility of the RRM domain, leading to a perfect exchange of two alpha-helices with an adjacent protein molecule in the crystal lattice.
Crystal structure of the RRM domain of poly(A)-specific ribonuclease reveals a novel m(7)G-cap-binding mode., Monecke T, Schell S, Dickmanns A, Ficner R, J Mol Biol. 2008 Oct 17;382(4):827-34. Epub 2008 Jul 31. PMID:18694759
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3CTR is a 1 chain structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Monecke T, Schell S, Dickmanns A, Ficner R. Crystal structure of the RRM domain of poly(A)-specific ribonuclease reveals a novel m(7)G-cap-binding mode. J Mol Biol. 2008 Oct 17;382(4):827-34. Epub 2008 Jul 31. PMID:18694759 doi:10.1016/j.jmb.2008.07.073
Page seeded by OCA on Tue Feb 17 17:49:48 2009
Categories: Homo sapiens | Dickmanns, A. | Ficner, R. | Monecke, T. | Schell, S. | Cytoplasm | Exonuclease | Hydrolase | M7g-cap | M7gtp | Magnesium | Metal-binding | Nonsense-mediated mrna decay | Nuclease | Nucleus | Parn | Phosphoprotein | Protein-rna-complex | Rna recognition motif | Rna-binding | Rrm
