First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
3cb4
From Proteopedia
Contents |
The Crystal Structure of LepA, the ribosomal back translocase
LepA is a highly conserved elongation factor that promotes the back translocation of tRNAs on the ribosome during the elongation cycle. We have determined the crystal structure of LepA from Escherichia coli at 2.8-A resolution. The high degree of sequence identity between LepA and EF-G is reflected in the structural similarity between the individual homologous domains of LepA and EF-G. However, the orientation of domains III and V in LepA differs from their orientations in EF-G. LepA also contains a C-terminal domain (CTD) not found in EF-G that has a previously unobserved protein fold. The high structural similarity between LepA and EF-G enabled us to derive a homology model for LepA bound to the ribosome using a 7.3-A cryo-EM structure of a complex between EF-G and the 70S ribosome. In this model, the very electrostatically positive CTD of LepA is placed in the direct vicinity of the A site of the large ribosomal subunit, suggesting a possible interaction between the CTD and the back translocated tRNA or 23S rRNA.
The structure of LepA, the ribosomal back translocase., Evans RN, Blaha G, Bailey S, Steitz TA, Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4673-8. Epub 2008 Mar 24. PMID:18362332
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
| 3cb4 asymmetric unit, resolution 2.80Å Click to display the biological molecule. | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene: | lepA (Escherichia coli K12) | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
About this Structure
The Basics
3cb4 is a 6 chains structure of sequences from Escherichia coli k12. Full crystallographic information is available from OCA.
This structure is used in an entry detailing LepA, the ribosomal back translocase, and can be found here.
Related Structures and Topics
- The Ribosomal Back Translocase topic page in Proteopedia
- Ef-G
- The Ribosome
- 70S ribosome bound to EF-G: 2wri , 2wrj, 2wrk, 2wrl
Reference
- Evans RN, Blaha G, Bailey S, Steitz TA. The structure of LepA, the ribosomal back translocase. Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4673-8. Epub 2008 Mar 24. PMID:18362332
Original page seeded by OCA on Wed Jun 3 08:45:42 2009
