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3bze
From Proteopedia
| 3bze, resolution 2.50Å () | |||||||||
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| Domains: | IGc, MHC_I | ||||||||
| Related: | 3bzf
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
The human non-classical major histocompatibility complex molecule HLA-E
Human leukocyte antigen (HLA)-E is a non-classical major histocompatibility complex class I molecule that binds peptides derived from the leader sequences of other HLA class I molecules. Natural killer cell recognition of these HLA-E molecules, via the CD94-NKG2 natural killer family, represents a central innate mechanism for monitoring major histocompatibility complex expression levels within a cell. The leader sequence-derived peptides bound to HLA-E exhibit very limited polymorphism, yet subtle differences affect the recognition of HLA-E by the CD94-NKG2 receptors. To better understand the basis for this peptide-specific recognition, we determined the structure of HLA-E in complex with two leader peptides, namely, HLA-Cw*07 (VMAPRALLL), which is poorly recognised by CD94-NKG2 receptors, and HLA-G*01 (VMAPRTLFL), a high-affinity ligand of CD94-NKG2 receptors. A comparison of these structures, both of which were determined to 2.5-A resolution, revealed that allotypic variations in the bound leader sequences do not result in conformational changes in the HLA-E heavy chain, although subtle changes in the conformation of the peptide within the binding groove of HLA-E were evident. Accordingly, our data indicate that the CD94-NKG2 receptors interact with HLA-E in a manner that maximises the ability of the receptors to discriminate between subtle changes in both the sequence and conformation of peptides bound to HLA-E.
Subtle changes in peptide conformation profoundly affect recognition of the non-classical MHC class I molecule HLA-E by the CD94-NKG2 natural killer cell receptors., Hoare HL, Sullivan LC, Clements CS, Ely LK, Beddoe T, Henderson KN, Lin J, Reid HH, Brooks AG, Rossjohn J, J Mol Biol. 2008 Apr 11;377(5):1297-303. Epub 2008 Feb 12. PMID:18339401
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3BZE is a 12 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Hoare HL, Sullivan LC, Clements CS, Ely LK, Beddoe T, Henderson KN, Lin J, Reid HH, Brooks AG, Rossjohn J. Subtle changes in peptide conformation profoundly affect recognition of the non-classical MHC class I molecule HLA-E by the CD94-NKG2 natural killer cell receptors. J Mol Biol. 2008 Apr 11;377(5):1297-303. Epub 2008 Feb 12. PMID:18339401 doi:10.1016/j.jmb.2008.01.098
Page seeded by OCA on Wed Feb 18 08:01:25 2009
Categories: Homo sapiens | Beddoe, T. | Brooks, A G. | Clements, C S. | Ely, L K. | Henderson, K N. | Hoare, H L. | Lin, J. | Reid, H H. | Rossjohn, J. | Sullivan, L C. | Disease mutation | Glycation | Glycoprotein | Immune response | Immune system | Immunoglobulin domain | Mhc fold | Mhc i | Pyrrolidone carboxylic acid | Secreted | Transmembrane
