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3l2c
From Proteopedia
| 3l2c, resolution 1.87Å () | |||||||||
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| Sites: | and | ||||||||
| Ligands: | |||||||||
| Gene: | AFX, AFX1, FOXO4, MLLT7 (Homo sapiens) | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal Structure of the DNA Binding Domain of FOXO4 Bound to DNA
FOXO4 is a member of the FOXO subgroup of forkhead transcription factors that constitute key components of a conserved signalling pathway that connects growth and stress signals to transcriptional control. Here, the 1.9 A resolution crystal structure of the DNA-binding domain of human FOXO4 (FOXO4-DBD) bound to a 13 bp DNA duplex containing a FOXO consensus binding sequence is reported. The structure shows a similar recognition of the core sequence as has been shown for two other FOXO proteins. Helix H3 is docked into the major groove and provides all of the base-specific contacts, while the N-terminus and wing W1 make additional contacts with the phosphate groups of DNA. In contrast to other FOXO-DBD-DNA structures, the loop between helices H2 and H3 has a different conformation and participates in DNA binding. In addition, the structure of the FOXO4-DBD-DNA complex suggests that both direct water-DNA base contacts and the unique water-network interactions contribute to FOXO-DBD binding to the DNA in a sequence-specific manner.
Structure of the human FOXO4-DBD-DNA complex at 1.9 A resolution reveals new details of FOXO binding to the DNA., Boura E, Rezabkova L, Brynda J, Obsilova V, Obsil T, Acta Crystallogr D Biol Crystallogr. 2010 Dec;66(Pt 12):1351-7. Epub 2010, Nov 16. PMID:21123876
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3L2C is a 3 chains structure with sequences from Homo sapiens. This structure supersedes the now removed PDB entry 3bpy. Full crystallographic information is available from OCA.
Reference
- Boura E, Rezabkova L, Brynda J, Obsilova V, Obsil T. Structure of the human FOXO4-DBD-DNA complex at 1.9 A resolution reveals new details of FOXO binding to the DNA. Acta Crystallogr D Biol Crystallogr. 2010 Dec;66(Pt 12):1351-7. Epub 2010, Nov 16. PMID:21123876 doi:10.1107/S0907444910042228
Page seeded by OCA on Wed Dec 22 09:03:15 2010
