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3bn3
From Proteopedia
| 3bn3, resolution 2.10Å () | |||||||||
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| Sites: | , , , , , , , and | ||||||||
| Ligands: | , , | ||||||||
| Gene: | ITGAL, CD11A (Homo sapiens), ICAM5, TLCN, TLN (Homo sapiens) | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
crystal structure of ICAM-5 in complex with aL I domain
Integrins are cell surface receptors that transduce signals bidirectionally across the plasma membrane. The key event of integrin signaling is the allosteric regulation between its ligand-binding site and the C-terminal helix (alpha7) of integrin's inserted (I) domain. A significant axial movement of the alpha7 helix is associated with the open, active conformation of integrins. We describe the crystal structure of an engineered high-affinity I domain from the integrin alpha(L)beta(2) (LFA-1) alpha subunit in complex with the N-terminal two domains of ICAM-5, an adhesion molecule expressed in telencephalic neurons. The finding that the alpha7 helix swings out and inserts into a neighboring I domain in an upside-down orientation in the crystals implies an intrinsically unusual mobility of this helix. This remarkable feature allows the alpha7 helix to trigger integrin's large-scale conformational changes with little energy penalty. It serves as a mechanistic example of how a weakly bound adhesion molecule works in signaling.
An unusual allosteric mobility of the c-terminal helix of a high-affinity alphaL integrin I domain variant bound to ICAM-5., Zhang H, Casasnovas JM, Jin M, Liu JH, Gahmberg CG, Springer TA, Wang JH, Mol Cell. 2008 Aug 8;31(3):432-7. PMID:18691975
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3BN3 is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Zhang H, Casasnovas JM, Jin M, Liu JH, Gahmberg CG, Springer TA, Wang JH. An unusual allosteric mobility of the C-terminal helix of a high-affinity alphaL integrin I domain variant bound to ICAM-5. Mol Cell. 2008 Aug 8;31(3):432-7. PMID:18691975 doi:10.1016/j.molcel.2008.06.022
Page seeded by OCA on Mon Feb 16 21:53:38 2009
