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3bbp
From Proteopedia
| 3bbp, resolution 3.00Å () | |||||||||
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| Sites: | , , , , and | ||||||||
| Ligands: | , | ||||||||
| Gene: | RAB6A, RAB6 (Homo sapiens), GCC2, KIAA0336, RANBP2L4 (Homo sapiens) | ||||||||
| Related: | 2gil | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Rab6-GTP:GCC185 Rab binding domain complex
GCC185 is a large coiled-coil protein at the trans Golgi network that is required for receipt of transport vesicles inbound from late endosomes and for anchoring noncentrosomal microtubules that emanate from the Golgi. Here, we demonstrate that recruitment of GCC185 to the Golgi is mediated by two Golgi-localized small GTPases of the Rab and Arl families. GCC185 binds Rab6, and mutation of residues needed for Rab binding abolishes Golgi localization. The crystal structure of Rab6 bound to the GCC185 Rab-binding domain reveals that Rab6 recognizes a two-fold symmetric surface on a coiled coil immediately adjacent to a C-terminal GRIP domain. Unexpectedly, Rab6 binding promotes association of Arl1 with the GRIP domain. We present a structure-derived model for dual GTPase membrane attachment that highlights the potential ability of Rab GTPases to reach binding partners at a significant distance from the membrane via their unstructured and membrane-anchored, hypervariable domains.
Rab and Arl GTPase family members cooperate in the localization of the golgin GCC185., Burguete AS, Fenn TD, Brunger AT, Pfeffer SR, Cell. 2008 Jan 25;132(2):286-98. PMID:18243103
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3BBP is a 6 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Burguete AS, Fenn TD, Brunger AT, Pfeffer SR. Rab and Arl GTPase family members cooperate in the localization of the golgin GCC185. Cell. 2008 Jan 25;132(2):286-98. PMID:18243103 doi:10.1016/j.cell.2007.11.048
Page seeded by OCA on Sun Mar 22 10:53:10 2009
Categories: Homo sapiens | Brunger, A T. | Burguete, A Schweizer. | Fenn, T D. | Pfeffer, S R. | Acetylation | Alternative splicing | Arl gtpase | Clasp protein | Coiled coil | Cytoplasm | Er-golgi transport | Golgi apparatus | Golgi complex | Golgin | Grip domain | Gtp-binding | Lipoprotein | Membrane | Methylation | Nucleotide-binding | Phosphorylation | Polymorphism | Prenylation | Protein transport | Protein transport/splicing complex | Rab effector | Rab gtpase | Transport
