|2zuo, resolution 3.50Å ()|
The structure of rat liver vault at 3.5 angstrom resolution
Vaults are among the largest cytoplasmic ribonucleoprotein particles and are found in numerous eukaryotic species. Roles in multidrug resistance and innate immunity have been suggested, but the cellular function remains unclear. We have determined the x-ray structure of rat liver vault at 3.5 angstrom resolution and show that the cage structure consists of a dimer of half-vaults, with each half-vault comprising 39 identical major vault protein (MVP) chains. Each MVP monomer folds into 12 domains: nine structural repeat domains, a shoulder domain, a cap-helix domain, and a cap-ring domain. Interactions between the 42-turn-long cap-helix domains are key to stabilizing the particle. The shoulder domain is structurally similar to a core domain of stomatin, a lipid-raft component in erythrocytes and epithelial cells.
The structure of rat liver vault at 3.5 angstrom resolution., Tanaka H, Kato K, Yamashita E, Sumizawa T, Zhou Y, Yao M, Iwasaki K, Yoshimura M, Tsukihara T, Science. 2009 Jan 16;323(5912):384-8. PMID:19150846
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2zuo is a 13 chain structure with sequence from Rattus norvegicus. The June 2009 RCSB PDB Molecule of the Month feature on Vaults by David Goodsell is 10.2210/rcsb_pdb/mom_2009_6. Full crystallographic information is available from OCA.
- Tanaka H, Kato K, Yamashita E, Sumizawa T, Zhou Y, Yao M, Iwasaki K, Yoshimura M, Tsukihara T. The structure of rat liver vault at 3.5 angstrom resolution. Science. 2009 Jan 16;323(5912):384-8. PMID:19150846 doi:http://dx.doi.org/323/5912/384
- Kato K, Tanaka H, Sumizawa T, Yoshimura M, Yamashita E, Iwasaki K, Tsukihara T. A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry. Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):525-31. Epub 2008, Apr 19. PMID:18453688 doi:http://dx.doi.org/10.1107/S0907444908004277