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2yev

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2yev, resolution 2.36Å ()
Ligands: , , , , , , , , ,
Non-Standard Residues:
Activity: Cytochrome-c oxidase, with EC number 1.9.3.1


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Structure of caa3-type cytochrome oxidase

Publication Abstract from PubMed

Cytochrome c oxidase is a member of the haem copper oxidase superfamily (HCO). HCOs function as the terminal enzymes in the respiratory chain of mitochondria and aerobic prokaryotes, coupling molecular oxygen reduction to transmembrane proton pumping. Integral to the enzyme's function is the transfer of electrons from cytochrome c to the oxidase via a transient association of the two proteins. Electron entry and exit are proposed to occur from the same site on cytochrome c. Here we report the crystal structure of the caa3-type cytochrome oxidase from Thermus thermophilus, which has a covalently tethered cytochrome c domain. Crystals were grown in a bicontinuous mesophase using a synthetic short-chain monoacylglycerol as the hosting lipid. From the electron density map, at 2.36 A resolution, a novel integral membrane subunit and a native glycoglycerophospholipid embedded in the complex were identified. Contrary to previous electron transfer mechanisms observed for soluble cytochrome c, the structure reveals the architecture of the electron transfer complex for the fused cupredoxin/cytochrome c domain, which implicates different sites on cytochrome c for electron entry and exit. Support for an alternative to the classical proton gate characteristic of this HCO class is presented.

Structural insights into electron transfer in caa3-type cytochrome oxidase., Lyons JA, Aragao D, Slattery O, Pisliakov AV, Soulimane T, Caffrey M, Nature. 2012 Jul 26;487(7408):514-8. PMID:22763450

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2yev is a 6 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

  • Lyons JA, Aragao D, Slattery O, Pisliakov AV, Soulimane T, Caffrey M. Structural insights into electron transfer in caa3-type cytochrome oxidase. Nature. 2012 Jul 26;487(7408):514-8. PMID:22763450 doi:10.1038/nature11182

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