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2y17
From Proteopedia
Contents |
EF-TU COMPLEX 3
The ribosome converts genetic information into protein by selecting aminoacyl tRNAs whose anticodons base-pair to an mRNA codon. Mutations in the tRNA body can perturb this process and affect fidelity. The Hirsh suppressor is a well-studied tRNA(Trp) harboring a G24A mutation that allows readthrough of UGA stop codons. Here we present crystal structures of the 70S ribosome complexed with EF-Tu and aminoacyl tRNA (native tRNA(Trp), G24A tRNA(Trp) or the miscoding A9C tRNA(Trp)) bound to cognate UGG or near-cognate UGA codons, determined at 3.2-A resolution. The A9C and G24A mutations lead to miscoding by facilitating the distortion of tRNA required for decoding. A9C accomplishes this by increasing tRNA flexibility, whereas G24A allows the formation of an additional hydrogen bond that stabilizes the distortion. Our results also suggest that each native tRNA will adopt a unique conformation when delivered to the ribosome that allows accurate decoding.
How mutations in tRNA distant from the anticodon affect the fidelity of decoding., Schmeing TM, Voorhees RM, Kelley AC, Ramakrishnan V, Nat Struct Mol Biol. 2011 Apr;18(4):432-6. Epub 2011 Mar 6. PMID:21378964
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2y17 is a 32 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein L1
- Ribosomal protein L10
- Ribosomal protein L15
- Ribosomal protein L16
- Ribosomal protein L17
- Ribosomal protein L18
- Ribosomal protein L19
- Ribosomal protein L2
- Ribosomal protein L20
- Ribosomal protein L21
- Ribosomal protein L22
- Ribosomal protein L23
- Ribosomal protein L24
- Ribosomal protein L25
- Ribosomal protein L27
- Ribosomal protein L28
- Ribosomal protein L29
- Ribosomal protein L3
- Ribosomal protein L30
- Ribosomal protein L31
- Ribosomal protein L32
- Ribosomal protein L33
- Ribosomal protein L34
- Ribosomal protein L35
- Ribosomal protein L36
- Ribosomal protein L4
- Ribosomal protein L5
- Ribosomal protein L6
Reference
- Schmeing TM, Voorhees RM, Kelley AC, Ramakrishnan V. How mutations in tRNA distant from the anticodon affect the fidelity of decoding. Nat Struct Mol Biol. 2011 Apr;18(4):432-6. Epub 2011 Mar 6. PMID:21378964 doi:10.1038/nsmb.2003
