2x25

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2x25, resolution 1.20Å ()
Non-Standard Residues:
Activity: Peptidylprolyl isomerase, with EC number 5.2.1.8
Related: 1vbs, 1oca, 1mf8, 2cyh, 1cwb, 1vbt, 1m9e, 1cwl, 1cwc, 1cwi, 1cwo, 1rmh, 1cwj, 2rmb, 1m9c, 1cwa, 1cwf, 1m9y, 3cyh, 1m9f, 4cyh, 1cwh, 1bck, 1w8v, 1awr, 1nmk, 1mik, 1awv, 1m9d, 1awt, 2cpl, 1fgl, 1m9x, 1m63, 1cwk, 5cyh, 1ak4, 3cys, 2alf, 1aws, 1w8l, 1awu, 1w8m, 2rma, 1cwm, 1awq, 2x2d, 2x2a, 2x2c


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

FREE ACETYL-CYPA ORTHORHOMBIC FORM

Publication Abstract from PubMed

Cyclophilin A (CypA) is a ubiquitous cis-trans prolyl isomerase with key roles in immunity and viral infection. CypA suppresses T-cell activation through cyclosporine complexation and is required for effective HIV-1 replication in host cells. We show that CypA is acetylated in diverse human cell lines and use a synthetically evolved acetyllysyl-tRNA synthetase/tRNA(CUA) pair to produce recombinant acetylated CypA in Escherichia coli. We determined atomic-resolution structures of acetylated CypA and its complexes with cyclosporine and HIV-1 capsid. Acetylation markedly inhibited CypA catalysis of cis to trans isomerization and stabilized cis rather than trans forms of the HIV-1 capsid. Furthermore, CypA acetylation antagonized the immunosuppressive effects of cyclosporine by inhibiting the sequential steps of cyclosporine binding and calcineurin inhibition. Our results reveal that acetylation regulates key functions of CypA in immunity and viral infection and provide a general set of mechanisms by which acetylation modulates interactions to regulate cell function.

Acetylation regulates cyclophilin A catalysis, immunosuppression and HIV isomerization., Lammers M, Neumann H, Chin JW, James LC, Nat Chem Biol. 2010 May;6(5):331-7. Epub 2010 Apr 4. PMID:20364129

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2x25 is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

See Also

Reference

  • Lammers M, Neumann H, Chin JW, James LC. Acetylation regulates cyclophilin A catalysis, immunosuppression and HIV isomerization. Nat Chem Biol. 2010 May;6(5):331-7. Epub 2010 Apr 4. PMID:20364129 doi:10.1038/nchembio.342

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