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2wlw

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2wlw, resolution 1.50Å ()
Activity: Peptidylprolyl isomerase, with EC number 5.2.1.8
Related: 2wlv
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


STRUCTURE OF THE N-TERMINAL CAPSID DOMAIN OF HIV-2

Publication Abstract from PubMed

TRIMCyps are primate antiretroviral proteins that potently inhibit HIV replication. Here we describe how rhesus macaque TRIMCyp (RhTC) has evolved to target and restrict HIV-2. We show that the ancestral cyclophilin A (CypA) domain of RhTC targets HIV-2 capsid with weak affinity, which is strongly increased in RhTC by two mutations (D66N and R69H) at the expense of HIV-1 binding. These mutations disrupt a constraining intramolecular interaction in CypA, triggering the complete restructuring (>16 A) of an active site loop. This new configuration discriminates between divergent HIV-1 and HIV-2 loop conformations mediated by capsid residue 88. Viral sensitivity to RhTC restriction can be conferred or abolished by mutating position 88. Furthermore, position 88 determines the susceptibility of naturally occurring HIV-1 sequences to restriction. Our results reveal the complex molecular, structural and thermodynamic changes that underlie the ongoing evolutionary race between virus and host.

Active site remodeling switches HIV specificity of antiretroviral TRIMCyp., Price AJ, Marzetta F, Lammers M, Ylinen LM, Schaller T, Wilson SJ, Towers GJ, James LC, Nat Struct Mol Biol. 2009 Oct;16(10):1036-42. Epub 2009 Sep 20. PMID:19767750

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2wlw is a 1 chain structure with sequence from Macaca mulatta. Full crystallographic information is available from OCA.

Reference

  • Price AJ, Marzetta F, Lammers M, Ylinen LM, Schaller T, Wilson SJ, Towers GJ, James LC. Active site remodeling switches HIV specificity of antiretroviral TRIMCyp. Nat Struct Mol Biol. 2009 Oct;16(10):1036-42. Epub 2009 Sep 20. PMID:19767750 doi:10.1038/nsmb.1667

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