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2vx8
From Proteopedia
| 2vx8, resolution 2.20Å () | |||||||||
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| Sites: | , and | ||||||||
| Ligands: | |||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
VAMP7 LONGIN DOMAIN HRB PEPTIDE COMPLEX
SNAREs provide the specificity and energy for the fusion of vesicles with their target membrane, but how they are sorted into the appropriate vesicles on post-Golgi trafficking pathways is largely unknown. We demonstrate that the clathrin-mediated endocytosis of the SNARE VAMP7 is directly mediated by Hrb, a clathrin adaptor and ArfGAP. Hrb wraps 20 residues of its unstructured C-terminal tail around the folded VAMP7 longin domain, demonstrating that unstructured regions of clathrin adaptors can select cargo. Disrupting this interaction by mutation of the VAMP7 longin domain or depletion of Hrb causes VAMP7 to accumulate on the cell's surface. However, the SNARE helix of VAMP7 binds back onto its longin domain, outcompeting Hrb for binding to the same groove and suggesting that Hrb-mediated endocytosis of VAMP7 occurs only when VAMP7 is incorporated into a cis-SNARE complex. These results elucidate the mechanism of retrieval of a postfusion SNARE complex in clathrin-coated vesicles.
Molecular basis for the sorting of the SNARE VAMP7 into endocytic clathrin-coated vesicles by the ArfGAP Hrb., Pryor PR, Jackson L, Gray SR, Edeling MA, Thompson A, Sanderson CM, Evans PR, Owen DJ, Luzio JP, Cell. 2008 Sep 5;134(5):817-27. PMID:18775314
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2VX8 is a 4 chains structure of sequences from Homo sapiens, mus musculus. Full crystallographic information is available from OCA.
Reference
- Pryor PR, Jackson L, Gray SR, Edeling MA, Thompson A, Sanderson CM, Evans PR, Owen DJ, Luzio JP. Molecular basis for the sorting of the SNARE VAMP7 into endocytic clathrin-coated vesicles by the ArfGAP Hrb. Cell. 2008 Sep 5;134(5):817-27. PMID:18775314 doi:10.1016/j.cell.2008.07.023
Page seeded by OCA on Tue Feb 17 05:50:45 2009
Categories: Homo sapiens, mus musculus | Evans, P R. | Luzio, J P. | Owen, D J. | Acetylation | Clathrin adaptor | Coiled coil | Cytoplasmic vesicle | Endocytosis | Endoplasmic reticulum | Endosome | Exocytosis | Golgi apparatus | Lysosome | Membrane | Membrane protein | Protein transport | Signal-anchor | Snare | Transmembrane | Transport | Vesicle transport
