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2vvz

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2vvz, resolution 2.50Å ()
Ligands: ,
Activity: Exo-alpha-sialidase, with EC number 3.2.1.18
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

STRUCTURE OF THE CATALYTIC DOMAIN OF STREPTOCOCCUS PNEUMONIAE SIALIDASE NANA

Publication Abstract from PubMed

Streptococcus pneumoniae genomes encode three sialidases, NanA, NanB and NanC, which are key virulence factors that remove sialic acids from various glycoconjugates. The enzymes have potential as drug targets and also as vaccine candidates. The 115 kDa NanA is the largest of the three sialidases and is anchored to the bacterial membrane. Although recombinantly expressed full-length NanA was soluble, it failed to crystallize; therefore, a 56.5 kDa domain that retained full enzyme activity was subcloned. The purified enzyme was crystallized in 0.1 M MES pH 6.5, 30%(w/v) PEG 4000 using the sitting-drop vapour-diffusion method. Data were collected at 100 K to 2.5 A resolution from a crystal grown in the presence of the inhibitor 2-deoxy-2,3-dehydro-N-acetyl neuraminic acid. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 49.2, b = 95.6, c = 226.6 A. The structure was solved by molecular replacement and refined to final R and R(free) factors of 0.246 and 0.298, respectively.

Structure of the catalytic domain of Streptococcus pneumoniae sialidase NanA., Xu G, Li X, Andrew PW, Taylor GL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Sep 1;64(Pt, 9):772-5. Epub 2008 Aug 20. PMID:18765901

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2vvz is a 2 chain structure with sequence from Streptococcus pneumoniae. Full crystallographic information is available from OCA.

See Also

Reference

  • Xu G, Li X, Andrew PW, Taylor GL. Structure of the catalytic domain of Streptococcus pneumoniae sialidase NanA. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Sep 1;64(Pt, 9):772-5. Epub 2008 Aug 20. PMID:18765901 doi:10.1107/S1744309108024044

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