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2vj2
From Proteopedia
| 2vj2, resolution 2.50Å () | |||||||||
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| Related: | 2cao | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
HUMAN JAGGED-1, DOMAINS DSL AND EGFS1-3
The Notch receptor and its ligands are key components in a core metazoan signaling pathway that regulates the spatial patterning, timing and outcome of many cell-fate decisions. Ligands contain a disulfide-rich Delta/Serrate/LAG-2 (DSL) domain required for Notch trans-activation or cis-inhibition. Here we report the X-ray structure of a receptor binding region of a Notch ligand, the DSL-EGF3 domains of human Jagged-1 (J-1(DSL-EGF3)). The structure reveals a highly conserved face of the DSL domain, and we show, by functional analysis of Drosophila melanogster ligand mutants, that this surface is required for both cis- and trans-regulatory interactions with Notch. We also identify, using NMR, a surface of Notch-1 involved in J-1(DSL-EGF3) binding. Our data imply that cis- and trans-regulation may occur through the formation of structurally distinct complexes that, unexpectedly, involve the same surfaces on both ligand and receptor.
A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition., Cordle J, Johnson S, Tay JZ, Roversi P, Wilkin MB, de Madrid BH, Shimizu H, Jensen S, Whiteman P, Jin B, Redfield C, Baron M, Lea SM, Handford PA, Nat Struct Mol Biol. 2008 Aug;15(8):849-57. Epub 2008 Jul 27. PMID:18660822
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2VJ2 is a 2 chains structure with sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Cordle J, Johnson S, Tay JZ, Roversi P, Wilkin MB, de Madrid BH, Shimizu H, Jensen S, Whiteman P, Jin B, Redfield C, Baron M, Lea SM, Handford PA. A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. Nat Struct Mol Biol. 2008 Aug;15(8):849-57. Epub 2008 Jul 27. PMID:18660822 doi:10.1038/nsmb.1457
Page seeded by OCA on Wed Aug 25 08:17:12 2010
Categories: Homo sapiens | Cordle, J. | Handford, P A. | Johnson, S. | Lea, S M. | Roversi, P. | Tay, J Z. | Developmental protein | Disease mutation | Dsl | Egf | Egf-like domain | Extracellular | Glycoprotein | Jagged | Membrane | Notch | Notch signaling pathway | Protein-binding | Signalling | Transmembrane
