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2rhi
From Proteopedia
| 2rhi, resolution 1.66Å () | |||||||||
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| Ligands: | , | ||||||||
| Non-Standard Residues: | |||||||||
| Gene: | L3MBTL, KIAA0681, L3MBT (Homo sapiens) | ||||||||
| Domains: | MBT | ||||||||
| Related: | 2rhu, 2rhx, 2rhy, 2rhz, 2ri2, 2ri3, 2ri5 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of the 3-MBT domain from human L3MBTL1 in complex with H1.5K27me2 at 1.66 angstrom
Human L3MBTL1, which contains three malignant brain tumor (MBT) repeats, binds monomethylated and dimethylated lysines, but not trimethylated lysines, in several histone sequence contexts. In crystal structures of L3MBTL1 complexes, the monomethyl- and dimethyllysines insert into a narrow and deep cavity of aromatic residue-lined pocket 2, while a proline ring inserts into shallower pocket 1. We have also engineered a single Y to E substitution within the aromatic cage of the BPTF PHD finger, resulting in a reversal of binding preference from trimethyl- to dimethyllysine in an H3K4 sequence context. In both the "cavity insertion" (L3MBTL1) and "surface groove" (PHD finger) modes of methyllysine recognition, a carboxylate group both hydrogen bonds and ion pairs to the methylammonium proton. Our structural and binding studies of these two modules provide insights into the molecular principles governing the decoding of lysine methylation states, thereby highlighting a methylation state-specific layer of histone mark readout impacting on epigenetic regulation.
Structural basis for lower lysine methylation state-specific readout by MBT repeats of L3MBTL1 and an engineered PHD finger., Li H, Fischle W, Wang W, Duncan EM, Liang L, Murakami-Ishibe S, Allis CD, Patel DJ, Mol Cell. 2007 Nov 30;28(4):677-91. PMID:18042461
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2RHI is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Li H, Fischle W, Wang W, Duncan EM, Liang L, Murakami-Ishibe S, Allis CD, Patel DJ. Structural basis for lower lysine methylation state-specific readout by MBT repeats of L3MBTL1 and an engineered PHD finger. Mol Cell. 2007 Nov 30;28(4):677-91. PMID:18042461 doi:10.1016/j.molcel.2007.10.023
Page seeded by OCA on Wed Feb 18 06:15:15 2009
Categories: Homo sapiens | Li, H. | Patel, D J. | Alternative splicing | Beta barrel | Chromatin regulator | Dimethyl-lysine | Dna-binding | Metal-binding | Nucleus | Protein-peptide complex | Repressor | Transcription | Transcription regulation | Transcription/nuclear protein complex | Zinc | Zinc-finger
