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2qfa
From Proteopedia
| 2qfa, resolution 1.40Å () | |||||||||
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| Ligands: | , | ||||||||
| Gene: | BIRC5, API4, IAP4 (Homo sapiens), CDCA8, PESCRG3 (Homo sapiens), INCENP (Homo sapiens) | ||||||||
| Domains: | BIR | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of a Survivin-Borealin-INCENP core complex
The chromosomal passenger complex (CPC) is a key regulator of chromosome segregation and cytokinesis. CPC functions are connected to its localization. The complex first localizes to centromeres and later associates with the central spindle and midbody. Survivin, Borealin, and INCENP are the three components of the CPC that regulate the activity and localization of its enzymatic component, the kinase Aurora B. We determined the 1.4 A resolution crystal structure of the regulatory core of the CPC, revealing that Borealin and INCENP associate with the helical domain of Survivin to form a tight three-helical bundle. We used siRNA rescue experiments with structure-based mutants to explore the requirements for CPC localization. We show that the intertwined structural interactions of the core components lead to functional interdependence. Association of the core "passenger" proteins creates a single structural unit, whose composite molecular surface presents conserved residues essential for central spindle and midbody localization.
Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together., Jeyaprakash AA, Klein UR, Lindner D, Ebert J, Nigg EA, Conti E, Cell. 2007 Oct 19;131(2):271-85. PMID:17956729
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2QFA is a 3 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Jeyaprakash AA, Klein UR, Lindner D, Ebert J, Nigg EA, Conti E. Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together. Cell. 2007 Oct 19;131(2):271-85. PMID:17956729 doi:10.1016/j.cell.2007.07.045
Page seeded by OCA on Tue Feb 17 20:58:16 2009
Categories: Homo sapiens | Conti, E. | Ebert, J. | Jeyaprakash, A A. | Klein, U R. | Lindner, D. | Nigg, E A. | Alternative splicing | Apoptosis | Cell cycle | Cell cycle/cell cycle/cell cycle complex | Cell division | Centromere | Chromosomal protein | Coiled coil | Cytoplasm | Long helix | Metal-binding | Microtubule | Mitosis | Nucleus | Phosphorylation | Polymorphism | Protease inhibitor | Protein complex | Thiol protease inhibitor | Three-helical-bundle | Zinc
