First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
2px9
From Proteopedia
| 2px9, 20 NMR models () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Domains: | Uba2_SUMO, UBCc | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
The intrinsic affinity between E2 and the Cys domain of E1 in Ubiquitin-like modifications
Ubiquitin-like modifications, which are carried out by similar biochemical mechanisms, regulate nearly every aspect of cellular function. Despite the recent advancements in characterizing their enzymology, our knowledge about the dynamic processes of these modifications is still fragmentary. In this study, we have uncovered an intrinsic affinity between the SUMO E2 and the Cys domain of SUMO E1. NMR studies in combination with paramagnetic spin labeling demonstrate that this interaction is mediated by previously unknown interfaces on both E1 and E2 and places the two catalytic Cys residues of the two enzymes in close proximity. Site-directed mutagenesis and enzymatic assays indicate that the interaction is fundamentally important for the transfer of SUMO from E1 to E2. Results from this study suggest that the interaction between E2 and the Cys domain of E1 participates in guiding the E2's translocation to E1's enzymatic active site in ubiquitin-like modifications.
The intrinsic affinity between E2 and the Cys domain of E1 in ubiquitin-like modifications., Wang J, Hu W, Cai S, Lee B, Song J, Chen Y, Mol Cell. 2007 Jul 20;27(2):228-37. PMID:17643372
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2PX9 is a 2 chains structure of sequences from Homo sapiens. Full experimental information is available from OCA.
Reference
- Wang J, Hu W, Cai S, Lee B, Song J, Chen Y. The intrinsic affinity between E2 and the Cys domain of E1 in ubiquitin-like modifications. Mol Cell. 2007 Jul 20;27(2):228-37. PMID:17643372 doi:10.1016/j.molcel.2007.05.023
Page seeded by OCA on Wed Feb 18 00:37:15 2009
Categories: Homo sapiens | Cai, S. | Chen, Y. | Hu, W D. | Lee, B. | Song, J. | Wang, J H. | E1 | E2 | Nmr | Paramagnetic spin-labeling | Protein-protein interaction | Sae2 | Sumo | Ubc9 | Ubiquitination
