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2pff

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2pff, resolution 4.00Å ()
Non-Standard Residues:
Activity: Fatty-acyl-CoA synthase, with EC number 2.3.1.86


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Structural Insights of Yeast Fatty Acid Synthase

Publication Abstract from PubMed

In yeast, the whole metabolic pathway for making 16- and 18-carbon fatty acids is carried out by fatty acid synthase, a 2.6 megadalton molecular-weight macromolecular assembly containing six copies of all eight catalytic centers. We have determined its crystal structure, which illuminates how this enzyme is initially activated and then carries out multiple steps of synthesis in each of six sterically isolated reaction chambers. Six of the catalytic sites are in the wall of the assembly facing an acyl carrier protein (ACP) bound to the ketoacyl synthase domain. Two-dimensional diffusion of substrates to the catalytic sites may be achieved by the electrostatically negative ACP swinging to each of the six electrostatically positive catalytic sites. The phosphopantetheinyl transferase domain lies outside the shell of the assembly, inaccessible to ACP that lies inside, suggesting that the attachment of the pantetheine arm to ACP must occur before complete assembly of the complex.

The crystal structure of yeast fatty acid synthase, a cellular machine with eight active sites working together., Lomakin IB, Xiong Y, Steitz TA, Cell. 2007 Apr 20;129(2):319-32. PMID:17448991

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2pff is a 9 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

See Also

Reference

  • Lomakin IB, Xiong Y, Steitz TA. The crystal structure of yeast fatty acid synthase, a cellular machine with eight active sites working together. Cell. 2007 Apr 20;129(2):319-32. PMID:17448991 doi:10.1016/j.cell.2007.03.013

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