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2pa5
From Proteopedia
| 2pa5, resolution 1.60Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||||
| Gene: | PTPN9 (Homo sapiens) | ||||||||
| Activity: | Protein-tyrosine-phosphatase, with EC number 3.1.3.48 | ||||||||
| Related: | 2hnq | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Crystal structure of human protein tyrosine phosphatase PTPN9
Protein tyrosine phosphatases (PTPs) play a critical role in regulating cellular functions by selectively dephosphorylating their substrates. Here we present 22 human PTP crystal structures that, together with prior structural knowledge, enable a comprehensive analysis of the classical PTP family. Despite their largely conserved fold, surface properties of PTPs are strikingly diverse. A potential secondary substrate-binding pocket is frequently found in phosphatases, and this has implications for both substrate recognition and development of selective inhibitors. Structural comparison identified four diverse catalytic loop (WPD) conformations and suggested a mechanism for loop closure. Enzymatic assays revealed vast differences in PTP catalytic activity and identified PTPD1, PTPD2, and HDPTP as catalytically inert protein phosphatases. We propose a "head-to-toe" dimerization model for RPTPgamma/zeta that is distinct from the "inhibitory wedge" model and that provides a molecular basis for inhibitory regulation. This phosphatome resource gives an expanded insight into intrafamily PTP diversity, catalytic activity, substrate recognition, and autoregulatory self-association.
Large-scale structural analysis of the classical human protein tyrosine phosphatome., Barr AJ, Ugochukwu E, Lee WH, King ON, Filippakopoulos P, Alfano I, Savitsky P, Burgess-Brown NA, Muller S, Knapp S, Cell. 2009 Jan 23;136(2):352-63. PMID:19167335
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2pa5 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.
See Also
Reference
- Barr AJ, Ugochukwu E, Lee WH, King ON, Filippakopoulos P, Alfano I, Savitsky P, Burgess-Brown NA, Muller S, Knapp S. Large-scale structural analysis of the classical human protein tyrosine phosphatome. Cell. 2009 Jan 23;136(2):352-63. PMID:19167335 doi:10.1016/j.cell.2008.11.038
Categories: Homo sapiens | Protein-tyrosine-phosphatase | Arrowsmith, C H. | Barr, A. | Bunkoczi, G. | Das, S. | Delft, F von. | Edwards, A. | Knapp, S. | Papagrigoriou, E. | Pike, A C.W. | SGC, Structural Genomics Consortium. | Salah, E. | Savitsky, P. | Sundstrom, M. | Turnbull, A. | Ugochukwu, E. | Uppenberg, J. | Weigelt, J. | Hydrolase | Meg2 | Protein tyrosine phosphatase | Ptpn9 | Sgc | Structural genomic | Structural genomics consortium
