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2ow8
From Proteopedia
Contents |
Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. This file, 2OW8, contains the 30S ribosome subunit, two tRNA, and mRNA molecules. 50S ribosome subunit is in the file 1VSA.
Our understanding of the mechanism of protein synthesis has undergone rapid progress in recent years as a result of low-resolution X-ray and cryo-EM structures of ribosome functional complexes and high-resolution structures of ribosomal subunits and vacant ribosomes. Here, we present the crystal structure of the Thermus thermophilus 70S ribosome containing a model mRNA and two tRNAs at 3.7 A resolution. Many structural details of the interactions between the ribosome, tRNA, and mRNA in the P and E sites and the ways in which tRNA structure is distorted by its interactions with the ribosome are seen. Differences between the conformations of vacant and tRNA-bound 70S ribosomes suggest an induced fit of the ribosome structure in response to tRNA binding, including significant changes in the peptidyl-transferase catalytic site.
Crystal structure of a 70S ribosome-tRNA complex reveals functional interactions and rearrangements., Korostelev A, Trakhanov S, Laurberg M, Noller HF, Cell. 2006 Sep 22;126(6):1065-77. Epub 2006 Sep 7. PMID:16962654
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2ow8 is a 23 chain structure of Ribosomal protein S10, Ribosomal protein S11, Ribosomal protein S12, Ribosomal protein S13, Ribosomal protein S15, Ribosomal protein S17, Ribosomal protein S2, Ribosomal protein S20, Ribosomal protein S3, Ribosomal protein S4, Ribosomal protein THX and Ribosome with sequence from Escherichia coli and Thermus thermophilus hb27. This structure supersedes the now removed PDB entry 2i1c. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein S10
- Ribosomal protein S11
- Ribosomal protein S12
- Ribosomal protein S13
- Ribosomal protein S15
- Ribosomal protein S17
- Ribosomal protein S2
- Ribosomal protein S20
- Ribosomal protein S3
- Ribosomal protein S4
- Ribosomal protein THX
- Ribosome
Reference
- Korostelev A, Trakhanov S, Laurberg M, Noller HF. Crystal structure of a 70S ribosome-tRNA complex reveals functional interactions and rearrangements. Cell. 2006 Sep 22;126(6):1065-77. Epub 2006 Sep 7. PMID:16962654 doi:10.1016/j.cell.2006.08.032
