2orv

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2orv, resolution 2.30Å ()
Ligands: ,
Gene: TK1 (Homo sapiens)
Activity: Thymidine kinase, with EC number 2.7.1.21
Related: 2orw
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

human Thymidine Kinase 1 in complex with TP4A

Publication Abstract from PubMed

Human thymidine kinase 1 (hTK1) and structurally related TKs from other organisms catalyze the initial phosphorylation step in the thymidine salvage pathway. Though ATP is known to be the preferred phosphoryl donor for TK1-like enzymes, its exact binding mode and effect on the oligomeric state has not been analyzed. Here we report the structures of hTK1 and of the Thermotoga maritima thymidine kinase (TmTK) in complex with the bisubstrate inhibitor TP4A. The TmTK-TP4A structure reveals that the adenosine moiety of ATP binds at the subunit interface of the homotetrameric enzyme and that the majority of the ATP-enzyme interactions occur between the phosphate groups and the P-loop. In the hTK1 structure the adenosine group of TP4A exhibited no electron density. This difference between hTK1 and TmTK is rationalized by a difference in the conformation of their quaternary structure. A more open conformation, as seen in the TmTK-TP4A complex structure, is required to provide space for the adenosine moiety. Our analysis supports the formation of an analogous open conformation in hTK1 upon ATP binding.

Binding of ATP to TK1-like enzymes is associated with a conformational change in the quaternary structure., Segura-Pena D, Lutz S, Monnerjahn C, Konrad M, Lavie A, J Mol Biol. 2007 May 25;369(1):129-41. Epub 2007 Mar 15. PMID:17407781

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2orv is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

See Also

Reference

  • Segura-Pena D, Lutz S, Monnerjahn C, Konrad M, Lavie A. Binding of ATP to TK1-like enzymes is associated with a conformational change in the quaternary structure. J Mol Biol. 2007 May 25;369(1):129-41. Epub 2007 Mar 15. PMID:17407781 doi:10.1016/j.jmb.2007.02.104

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