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2o8f

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This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

2o8f, resolution 3.25Å ()

Ligands:

Gene:

MSH2 (Homo sapiens), MSH6, GTBP (Homo sapiens)

Domains:

ABC_MSH6_euk, MutS_II, MutS_I, MUTSd, MutS, MutS_III, ABC_MSH2_euk

Related:

2o8b, 2o8c, 2o8e, 2o8d

Structural annotation:
Resources:	InterPro : Ipr007696, Ipr011184, Ipr007861, Ipr000432, Ipr007860, Ipr007695, Ipr000313, Ipr015536 Pfam : PF05188, PF00488, PF05190, PF05192, PF01624 UniProt : P43246, P52701

Functional annotation:
Resources:	GeneCard : MSH2, MSH6
Cellular component:	MutSbeta complex nucleus MutSalpha complex nuclear chromatin
Biological process:	cell cycle response to X-ray DNA repair isotype switching B cell mediated immunity negative regulation of cell cycle response to DNA damage stimulus cell cycle arrest apoptosis base-excision repair in utero embryonic development intra-S DNA damage checkpoint somatic recombination of immunoglobulin gene segments oxidative phosphorylation cell motility negative regulation of neuron apoptosis B cell differentiation determination of adult life span negative regulation of DNA recombination purine nucleotide biosynthetic process postreplication repair somatic hypermutation of immunoglobulin genes mismatch repair maintenance of DNA repeat elements response to UV-B DNA damage response, signal transduction resulting in induction of apoptosis response to UV induction of apoptosis by intracellular signals
Molecular function:	magnesium ion binding nucleotide binding guanine/thymine mispair binding ADP binding DNA binding centromeric DNA binding mispaired DNA binding damaged DNA binding double-stranded DNA binding dinucleotide insertion or deletion binding dinucleotide repeat insertion binding single guanine insertion binding ATP binding protein binding single-stranded DNA binding four-way junction DNA binding hypoxanthine phosphoribosyltransferase activity mismatched DNA binding protein homodimerization activity MutLalpha complex binding oxidized purine DNA binding ATPase activity single thymine insertion binding identical protein binding chromatin binding purine-specific mismatch base pair DNA N-glycosylase activity

Evolutionary conservation:

Toggle Conservation Colors:

Rows = identical sequences:

Further Information:

Caveats,

Explanation,
Complete Results at ConSurfDB

Resources:

FirstGlance, OCA, PDBsum, RCSB

Coordinates:

save as pdb, mmCIF, xml

1 human MutSalpha (MSH2/MSH6) bound to DNA with a single base T insert
2 Disease
3 About this Structure
4 Reference

human MutSalpha (MSH2/MSH6) bound to DNA with a single base T insert

Publication Abstract from PubMed

Mismatch repair (MMR) ensures the fidelity of DNA replication, initiates the cellular response to certain classes of DNA damage, and has been implicated in the generation of immune diversity. Each of these functions depends on MutSalpha (MSH2*MSH6 heterodimer). Inactivation of this protein complex is responsible for tumor development in about half of known hereditary nonpolyposis colorectal cancer kindreds and also occurs in sporadic tumors in a variety of tissues. Here, we describe a series of crystal structures of human MutSalpha bound to different DNA substrates, each known to elicit one of the diverse biological responses of the MMR pathway. All lesions are recognized in a similar manner, indicating that diversity of MutSalpha-dependent responses to DNA lesions is generated in events downstream of this lesion recognition step. This study also allows rigorous mapping of cancer-causing mutations and furthermore suggests structural pathways for allosteric communication between different regions within the heterodimer.

Structure of the human MutSalpha DNA lesion recognition complex., Warren JJ, Pohlhaus TJ, Changela A, Iyer RR, Modrich PL, Beese LS, Mol Cell. 2007 May 25;26(4):579-92. PMID:17531815

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Disease

Known disease associated with this structure: Colorectal cancer, hereditary nonpolyposis, type 1 OMIM:[609309], Mismatch repair cancer syndrome OMIM:[609309], Muir-Torre syndrome OMIM:[609309]

About this Structure

2O8F is a 4 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Warren JJ, Pohlhaus TJ, Changela A, Iyer RR, Modrich PL, Beese LS. Structure of the human MutSalpha DNA lesion recognition complex. Mol Cell. 2007 May 25;26(4):579-92. PMID:17531815 doi:10.1016/j.molcel.2007.04.018

Page seeded by OCA on Mon Feb 16 19:44:56 2009

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Retrieved from "http://www.proteopedia.org/wiki/index.php/2o8f"

2o8f

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Contents

human MutSalpha (MSH2/MSH6) bound to DNA with a single base T insert

Disease

About this Structure

Reference

Proteopedia Page Contributors and Editors (what is this?)

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