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2nny
From Proteopedia
| 2nny, resolution 2.58Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene: | ETS1 (Homo sapiens) | ||||||||
| Domains: | Ets | ||||||||
| Related: | 1k78, 1gvj | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of the Ets1 dimer DNA complex.
The function of the Ets-1 transcription factor is regulated by two regions that flank its DNA-binding domain. A previously established mechanism for auto-inhibition of monomeric Ets-1 on DNA response elements with a single ETS-binding site, however, has not been observed for the stromelysin-1 promoter containing two palindromic ETS-binding sites. We present the structure of Ets-1 on this promoter element, revealing a ternary complex in which protein homo-dimerization is mediated by the specific arrangement of the two ETS-binding sites. In this complex, the N-terminal-flanking region is required for ternary protein-DNA assembly. Ets-1 variants, in which residues from this region are mutated, loose the ability for DNA-mediated dimerization and stromelysin-1 promoter transactivation. Thus, our data unravel the molecular basis for relief of auto-inhibition and the ability of Ets-1 to function as a facultative dimeric transcription factor on this site. Our findings may also explain previous data of Ets-1 function in the context of heterologous transcription factors, thus providing a molecular model that could also be valid for Ets-1 regulation by hetero-oligomeric assembly.
Regulation of the transcription factor Ets-1 by DNA-mediated homo-dimerization., Lamber EP, Vanhille L, Textor LC, Kachalova GS, Sieweke MH, Wilmanns M, EMBO J. 2008 Jul 23;27(14):2006-17. Epub 2008 Jun 19. PMID:18566588
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2NNY is a 4 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Lamber EP, Vanhille L, Textor LC, Kachalova GS, Sieweke MH, Wilmanns M. Regulation of the transcription factor Ets-1 by DNA-mediated homo-dimerization. EMBO J. 2008 Jul 23;27(14):2006-17. Epub 2008 Jun 19. PMID:18566588 doi:10.1038/emboj.2008.117
Page seeded by OCA on Tue Feb 17 17:43:22 2009
