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2nml
From Proteopedia
| 2nml, resolution 1.55Å () | |||||||||
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| Gene: | ERH (Homo sapiens) | ||||||||
| Domains: | ER | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of HEF2/ERH at 1.55 A resolution
Functional complementation screens can identify known or novel proteins with important intracellular activities. We have isolated human enhancer of filamentation 2 (HEF2) in a screen to find human genes that promote pseudohyphal growth in budding yeast. HEF2 is identical to enhancer of rudimentary homolog (ERH), a highly conserved protein of 104 amino acids. In silico protein-interaction mapping implies that HEF2/ERH interacts with transcription factors, cell-cycle regulators, and other proteins shown to enhance filamentous growth in S. cerevisiae, suggesting a context for studies of HEF2/ERH function. To provide a mechanistic basis to study of HEF2/ERH, we have determined the crystal structure of HEF2/ERH at 1.55 A. The crystal asymmetric unit contains a HEF2/ERH monomer. The two monomers of the physiological dimer are related by the y, x, -z crystal symmetric operation. The HEF2/ERH structure is characterized by a novel alpha + beta fold, a four-strand antiparallel beta-sheet with three alpha-helixes on one side of the sheet. The beta-sheets from the two monomers together constitute a pseudo-beta-barrel, and form the center of the functional HEF2/ERH dimer, with a cavity channel at the dimer interface. Docking of this structure to the HEF2/ERH partner protein DCOH/PCD suggests that HEF2/ERH may regulate the oligomeric state of this protein. These data suggest that HEF2/ERH may be an important transcription regulator that also functions in the control of cell-cycle progression.
A 1.55 A resolution X-ray crystal structure of HEF2/ERH and insights into its transcriptional and cell-cycle interaction networks., Jin T, Guo F, Serebriiskii IG, Howard A, Zhang YZ, Proteins. 2007 Aug 1;68(2):427-37. PMID:17444515
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2NML is a 1 chain structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 2i4f. Full crystallographic information is available from OCA.
Reference
- Jin T, Guo F, Serebriiskii IG, Howard A, Zhang YZ. A 1.55 A resolution X-ray crystal structure of HEF2/ERH and insights into its transcriptional and cell-cycle interaction networks. Proteins. 2007 Aug 1;68(2):427-37. PMID:17444515 doi:10.1002/prot.21343
Page seeded by OCA on Tue Feb 17 09:45:16 2009
