2kbu

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2kbu, 15 NMR models ()
Non-Standard Residues:
Activity: Peptidylprolyl isomerase, with EC number 5.2.1.8
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



NMR solution structure of Pin1 WW domain mutant with beta turn mimic at position 12

Publication Abstract from PubMed

beta-Turns are common conformations that enable proteins to adopt globular structures, and their formation is often rate limiting for folding. beta-Turn mimics, molecules that replace the i + 1 and i + 2 amino acid residues of a beta-turn, are envisioned to act as folding nucleators by preorganizing the pendant polypeptide chains, thereby lowering the activation barrier for beta-sheet formation. However, the crucial kinetic experiments to demonstrate that beta-turn mimics can act as strong nucleators in the context of a cooperatively folding protein have not been reported. We have incorporated 6 beta-turn mimics simulating varied beta-turn types in place of 2 residues in an engineered beta-turn 1 or beta-bulge turn 1 of the Pin 1 WW domain, a three-stranded beta-sheet protein. We present 2 lines of kinetic evidence that the inclusion of beta-turn mimics alters beta-sheet folding rates, enabling us to classify beta-turn mimics into 3 categories: those that are weak nucleators but permit Pin WW folding, native-like nucleators, and strong nucleators. Strong nucleators accelerate folding relative to WW domains incorporating all alpha-amino acid sequences. A solution NMR structure reveals that the native Pin WW beta-sheet structure is retained upon incorporating a strong E-olefin nucleator. These beta-turn mimics can now be used to interrogate protein folding transition state structures and the 2 kinetic analyses presented can be used to assess the nucleation capacity of other beta-turn mimics.

Evaluating {beta}-turn mimics as {beta}-sheet folding nucleators., Fuller AA, Du D, Liu F, Davoren JE, Bhabha G, Kroon G, Case DA, Dyson HJ, Powers ET, Wipf P, Gruebele M, Kelly JW, Proc Natl Acad Sci U S A. 2009 Jun 18. PMID:19541614

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2kbu is a 1 chain structure. Full experimental information is available from OCA.

Reference

  • Fuller AA, Du D, Liu F, Davoren JE, Bhabha G, Kroon G, Case DA, Dyson HJ, Powers ET, Wipf P, Gruebele M, Kelly JW. Evaluating {beta}-turn mimics as {beta}-sheet folding nucleators. Proc Natl Acad Sci U S A. 2009 Jun 18. PMID:19541614

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