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2hvj
From Proteopedia
| 2hvj, resolution 2.75Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||||
| Gene: | kcsA (Streptomyces lividans) | ||||||||
| Domains: | IGv, V-set, IGc, Ion_trans_2 | ||||||||
| Related: | 1k4d | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of KcsA-Fab-TBA complex in low K+
K(+) channels play essential roles in regulating membrane excitability of many diverse cell types by selectively conducting K(+) ions through their pores. Many diverse molecules can plug the pore and modulate the K(+) current. Quaternary ammonium (QA) ions are a class of pore blockers that have been used for decades by biophysicists to probe the pore, leading to important insights into the structure-function relation of K(+) channels. However, many key aspects of the QA-blocking mechanisms remain unclear to date, and understanding these questions requires high resolution structural information. Here, we address the question of whether intracellular QA blockade causes conformational changes of the K(+) channel selectivity filter. We have solved the structures of the KcsA K(+) channel in complex with tetrabutylammonium (TBA) and tetrabutylantimony (TBSb) under various ionic conditions. Our results demonstrate that binding of TBA or TBSb causes no significant change in the KcsA structure at high concentrations of permeant ions. We did observe the expected conformational change of the filter at low concentration of K(+), but this change appears to be independent of TBA or TBSb blockade.
Crystallographic study of the tetrabutylammonium block to the KcsA K+ channel., Yohannan S, Hu Y, Zhou Y, J Mol Biol. 2007 Feb 23;366(3):806-14. Epub 2006 Dec 2. PMID:17196615
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2HVJ is a 3 chains structure of sequences from Mus musculus and Streptomyces lividans. Full crystallographic information is available from OCA.
Reference
- Yohannan S, Hu Y, Zhou Y. Crystallographic study of the tetrabutylammonium block to the KcsA K+ channel. J Mol Biol. 2007 Feb 23;366(3):806-14. Epub 2006 Dec 2. PMID:17196615 doi:10.1016/j.jmb.2006.11.081
Page seeded by OCA on Tue Feb 17 08:51:54 2009
