First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
2gy9
From Proteopedia
Contents |
Structure of the 30S subunit of a pre-translocational E. coli ribosome obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1056
In E. coli, the SecM nascent polypeptide causes elongation arrest, while interacting with 23S RNA bases A2058 and A749-753 in the exit tunnel of the large ribosomal subunit. We compared atomic models fitted by real-space refinement into cryo-electron microscopy reconstructions of a pretranslocational and SecM-stalled E. coli ribosome complex. A cascade of RNA rearrangements propagates from the exit tunnel throughout the large subunit, affecting intersubunit bridges and tRNA positions, which in turn reorient small subunit RNA elements. Elongation arrest could result from the inhibition of mRNA.(tRNAs) translocation, E site tRNA egress, and perhaps translation factor activation at the GTPase-associated center. Our study suggests that the specific secondary and tertiary arrangement of ribosomal RNA provides the basis for internal signal transduction within the ribosome. Thus, the ribosome may itself have the ability to regulate its progression through translation by modulating its structure and consequently its receptivity to activation by cofactors.
Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements., Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J, Mol Cell. 2006 May 19;22(4):533-43. PMID:16713583
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2gy9 is a 23 chain structure of Ribosomal protein S10, Ribosomal protein S11, Ribosomal protein S12, Ribosomal protein S13, Ribosomal protein S14, Ribosomal protein S15, Ribosomal protein S16, Ribosomal protein S17, Ribosomal protein S18, Ribosomal protein S19, Ribosomal protein S2, Ribosomal protein S20, Ribosomal protein S3, Ribosomal protein S4, Ribosomal protein S5, Ribosomal protein S6, Ribosomal protein S7, Ribosomal protein S8, Ribosomal protein S9 and Ribosome with sequence from Escherichia coli. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein S10
- Ribosomal protein S11
- Ribosomal protein S12
- Ribosomal protein S13
- Ribosomal protein S14
- Ribosomal protein S15
- Ribosomal protein S16
- Ribosomal protein S17
- Ribosomal protein S18
- Ribosomal protein S19
- Ribosomal protein S2
- Ribosomal protein S20
- Ribosomal protein S3
- Ribosomal protein S4
- Ribosomal protein S5
- Ribosomal protein S6
- Ribosomal protein S7
- Ribosomal protein S8
- Ribosomal protein S9
- Ribosome
Reference
- Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J. Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements. Mol Cell. 2006 May 19;22(4):533-43. PMID:16713583 doi:10.1016/j.molcel.2006.05.003
- Mitra K, Frank J. A model for co-translational translocation: ribosome-regulated nascent polypeptide translocation at the protein-conducting channel. FEBS Lett. 2006 Jun 12;580(14):3353-60. Epub 2006 May 15. PMID:16714018 doi:10.1016/j.febslet.2006.05.019
