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2gtg
From Proteopedia
| 2gtg, resolution 2.40Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene: | PSAP (Homo sapiens) | ||||||||
| Domains: | SapB, SapB_1 | ||||||||
| Related: | 1n69, 1m12, 2dob | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Crystal Structure of Human Saposin C
Saposins A and C are sphingolipid activator proteins required for the lysosomal breakdown of galactosylceramide and glucosylceramide, respectively. The saposins interact with lipids, leading to an enhanced accessibility of the lipid headgroups to their cognate hydrolases. We have determined the crystal structures of human saposins A and C to 2.0 Angstroms and 2.4 Angstroms, respectively, and both reveal the compact, monomeric saposin fold. We confirmed that these two proteins were monomeric in solution at pH 7.0 by analytical centrifugation. However, at pH 4.8, in the presence of the detergent C(8)E(5), saposin A assembled into dimers, while saposin C formed trimers. Saposin B was dimeric under all conditions tested. The self-association of the saposins is likely to be relevant to how these small proteins interact with lipids, membranes, and hydrolase enzymes.
Crystal structures of saposins A and C., Ahn VE, Leyko P, Alattia JR, Chen L, Prive GG, Protein Sci. 2006 Aug;15(8):1849-57. Epub 2006 Jul 5. PMID:16823039
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
Disease
Known disease associated with this structure: Combined SAP deficiency OMIM:[176801], Gaucher disease, atypical OMIM:[176801], Krabbe disease, atypical OMIM:[176801], Metachromatic leukodystrophy due to SAP-b deficiency OMIM:[176801]
About this Structure
2GTG is a 1 chain structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Ahn VE, Leyko P, Alattia JR, Chen L, Prive GG. Crystal structures of saposins A and C. Protein Sci. 2006 Aug;15(8):1849-57. Epub 2006 Jul 5. PMID:16823039 doi:10.1110/ps.062256606
Page seeded by OCA on Mon Feb 16 20:47:05 2009
