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2g4b
From Proteopedia
| 2g4b, resolution 2.50Å () | |||||||||
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| Ligands: | |||||||||
| Gene: | U2AF65 (Homo sapiens) | ||||||||
| Related: | 2fzr | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Structure of U2AF65 variant with polyuridine tract
The essential pre-mRNA splicing factor, U2AF(65), guides the early stages of splice site choice by recognizing a polypyrimidine (Py) tract consensus sequence near the 3' splice site. Since Py tracts are relatively poorly conserved in higher eukaryotes, U2AF(65) is faced with the problem of specifying uridine-rich sequences, yet tolerating a variety of nucleotide substitutions found in natural Py tracts. To better understand these apparently contradictory RNA binding characteristics, the X-ray structure of the U2AF(65) RNA binding domain bound to a Py tract composed of seven uridines has been determined at 2.5 A resolution. Specific hydrogen bonds between U2AF(65) and the uracil bases provide an explanation for polyuridine recognition. Flexible side chains and bound water molecules form the majority of the base contacts and potentially could rearrange when the U2AF(65) structure adapts to different Py tract sequences. The energetic importance of conserved residues for Py tract binding is established by analysis of site-directed mutant U2AF(65) proteins using surface plasmon resonance.
Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65., Sickmier EA, Frato KE, Shen H, Paranawithana SR, Green MR, Kielkopf CL, Mol Cell. 2006 Jul 7;23(1):49-59. PMID:16818232
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2g4b is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Sickmier EA, Frato KE, Shen H, Paranawithana SR, Green MR, Kielkopf CL. Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65. Mol Cell. 2006 Jul 7;23(1):49-59. PMID:16818232 doi:10.1016/j.molcel.2006.05.025
- Sickmier EA, Frato KE, Kielkopf CL. Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 May 1;62(Pt, 5):457-9. Epub 2006 Apr 12. PMID:16682775 doi:10.1107/S1744309106012504
