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2f5n

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2f5n, resolution 2.00Å ()
Ligands: ,
Activity: DNA-formamidopyrimidine glycosylase, with EC number 3.2.2.23
Related: 2f5o, 2f5p, 2f5q, 2f5s
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

MutM crosslinked to undamaged DNA sampling A:T base pair IC1

Publication Abstract from PubMed

DNA glycosylases must interrogate millions of base pairs of undamaged DNA in order to locate and then excise one damaged nucleobase. The nature of this search process remains poorly understood. Here we report the use of disulfide cross-linking (DXL) technology to obtain structures of a bacterial DNA glycosylase, MutM, interrogating undamaged DNA. These structures, solved to 2.0 angstrom resolution, reveal the nature of the search process: The protein inserts a probe residue into the helical stack and severely buckles the target base pair, which remains intrahelical. MutM therefore actively interrogates the intact DNA helix while searching for damage.

Structure of a DNA glycosylase searching for lesions., Banerjee A, Santos WL, Verdine GL, Science. 2006 Feb 24;311(5764):1153-7. PMID:16497933

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2f5n is a 3 chain structure with sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

See Also

Reference

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