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2eyq
From Proteopedia
Crystal structure of Escherichia coli transcription-repair coupling factor
Coupling of transcription and DNA repair in bacteria is mediated by transcription-repair coupling factor (TRCF, the product of the mfd gene), which removes transcription elongation complexes stalled at DNA lesions and recruits the nucleotide excision repair machinery to the site. Here we describe the 3.2 A-resolution X-ray crystal structure of Escherichia coli TRCF. The structure consists of a compact arrangement of eight domains, including a translocation module similar to the SF2 ATPase RecG, and a region of structural similarity to UvrB. Biochemical and genetic experiments establish that another domain with structural similarity to the Tudor-like domain of the transcription elongation factor NusG plays a critical role in TRCF/RNA polymerase interactions. Comparison with the translocation module of RecG as well as other structural features indicate that TRCF function involves large-scale conformational changes. These data, along with a structural model for the interaction of TRCF with the transcription elongation complex, provide mechanistic insights into TRCF function.
Structural basis for bacterial transcription-coupled DNA repair., Deaconescu AM, Chambers AL, Smith AJ, Nickels BE, Hochschild A, Savery NJ, Darst SA, Cell. 2006 Feb 10;124(3):507-20. PMID:16469698
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
| 2eyq asymmetric unit, resolution 3.20Å. Click to display biological molecule. | |||||||||
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| Ligands: | , | ||||||||
| Gene: | mfd (Escherichia coli) | ||||||||
| Domains: | TRCF, CarD_TRCF, DEXHc, HELICc, PRK10689 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
2EYQ is a 2 chains structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Size exclusion chromatogaphy indicates that the protein
an N→C rainbow similar to Figure 1A of the article describing the structure. .
The domains in the structure of Mfd:
similar to Figure 1B and Figure 2 of the article describing the structure.
Figure 1B and Figure 2 of the article describing the structure. .
[Note: the following view generates a substantial surface which may take several minutes to calculate. Use the one above as an alternative unless you are willing to spend the time.] Figure 1B. .
displayed as in Figure 3A of the article describing the structure.
are highlighted in red. See figure 3B.
displayed similar to Figure 3B of the article describing the structure.
indicated similar to Figure 3C of the article describing the structure.
Reference
- Deaconescu AM, Chambers AL, Smith AJ, Nickels BE, Hochschild A, Savery NJ, Darst SA. Structural basis for bacterial transcription-coupled DNA repair. Cell. 2006 Feb 10;124(3):507-20. PMID:16469698 doi:10.1016/j.cell.2005.11.045
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