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2b9p
From Proteopedia
| 2b9p, resolution 6.46Å () | |||||||||
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| Related: | 2b64, 2b66, 2b9m, 2b9n, 2b9o | ||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
50S ribosomal subunit from a crystal structure of the ribosome in complex with tRNAs and mRNA with a stop codon in the A-site. This file contains the 50S subunit from a crystal structure of the ribosome in complex with tRNAs and mRNA with a stop codon in the A-site and is described in remark 400.
During protein synthesis, translational release factors catalyze the release of the polypeptide chain when a stop codon on the mRNA reaches the A site of the ribosome. The detailed mechanism of this process is currently unknown. We present here the crystal structures of the ribosome from Thermus thermophilus with RF1 and RF2 bound to their cognate stop codons, at resolutions of 5.9 Angstrom and 6.7 Angstrom, respectively. The structures reveal details of interactions of the factors with the ribosome and mRNA, including elements previously implicated in decoding and peptide release. They also shed light on conformational changes both in the factors and in the ribosome during termination. Differences seen in the interaction of RF1 and RF2 with the L11 region of the ribosome allow us to rationalize previous biochemical data. Finally, this work demonstrates the feasibility of crystallizing ribosomes with bound factors at a defined state along the translational pathway.
Crystal structures of the ribosome in complex with release factors RF1 and RF2 bound to a cognate stop codon., Petry S, Brodersen DE, Murphy FV 4th, Dunham CM, Selmer M, Tarry MJ, Kelley AC, Ramakrishnan V, Cell. 2005 Dec 29;123(7):1255-66. PMID:16377566
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2b9p is a 30 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein L11
- Ribosomal protein L13
- Ribosomal protein L14
- Ribosomal protein L15
- Ribosomal protein L16
- Ribosomal protein L17
- Ribosomal protein L18
- Ribosomal protein L19
- Ribosomal protein L2
- Ribosomal protein L20
- Ribosomal protein L21
- Ribosomal protein L22
- Ribosomal protein L23
- Ribosomal protein L27
- Ribosomal protein L29
- Ribosomal protein L3
- Ribosomal protein L30
- Ribosomal protein L31
- Ribosomal protein L32
- Ribosomal protein L33
- Ribosomal protein L34
- Ribosomal protein L35
- Ribosomal protein L36
- Ribosomal protein L4
- Ribosomal protein L5
- Ribosomal protein L6
- Ribosomal protein L9
Reference
- Petry S, Brodersen DE, Murphy FV 4th, Dunham CM, Selmer M, Tarry MJ, Kelley AC, Ramakrishnan V. Crystal structures of the ribosome in complex with release factors RF1 and RF2 bound to a cognate stop codon. Cell. 2005 Dec 29;123(7):1255-66. PMID:16377566 doi:10.1016/j.cell.2005.09.039
