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2aze

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This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

2aze, resolution 2.55Å ()

Gene:

TFDP1, DP1 (Homo sapiens), E2F1, RBBP3 (Homo sapiens), RB1 (Homo sapiens)

Domains:

DP, Rb_C

Structural annotation:
Resources:	InterPro : Ipr003316, Ipr011991, Ipr015648, Ipr014889, Ipr015633, Ipr015634, Ipr006670, Ipr011028, Ipr002720, Ipr015030, Ipr002719, Ipr013763, Ipr015652 Pfam : PF08781, PF08934 UniProt : Q14186, Q01094, P06400

Functional annotation:
Resources:	GeneCard : TFDP1, E2F1, RB1
Cellular component:	nucleus transcription factor complex cytoplasm chromatin PML body
Biological process:	cell cycle transcription regulation of transcription, DNA-dependent regulation of transcription from RNA polymerase II promoter cell proliferation positive regulation of transcription from RNA polymerase II promoter G1 phase of mitotic cell cycle forebrain development negative regulation of transcription from RNA polymerase II promoter apoptosis chromatin modification cell cycle checkpoint G1 phase M phase regulation of lipid kinase activity androgen receptor signaling pathway negative regulation of cell growth negative regulation of cell cycle positive regulation of transcription, DNA-dependent negative regulation of protein kinase activity
Molecular function:	DNA binding transcription factor activity transcription coactivator activity transcription activator activity protein binding transcription corepressor activity kinase binding androgen receptor binding

Evolutionary conservation:

Toggle Conservation Colors:

Rows = identical sequences:

Further Information:

Caveats,

Explanation,
Complete Results at ConSurfDB

Resources:

FirstGlance, OCA, PDBsum, RCSB

Coordinates:

save as pdb, mmCIF, xml

1 Structure of the Rb C-terminal domain bound to an E2F1-DP1 heterodimer
2 Disease
3 About this Structure
4 Reference

Structure of the Rb C-terminal domain bound to an E2F1-DP1 heterodimer

Publication Abstract from PubMed

The retinoblastoma (Rb) protein negatively regulates the G1-S transition by binding to the E2F transcription factors, until cyclin-dependent kinases phosphorylate Rb, causing E2F release. The Rb pocket domain is necessary for E2F binding, but the Rb C-terminal domain (RbC) is also required for growth suppression. Here we demonstrate a high-affinity interaction between RbC and E2F-DP heterodimers shared by all Rb and E2F family members. The crystal structure of an RbC-E2F1-DP1 complex reveals an intertwined heterodimer in which the marked box domains of both E2F1 and DP1 contact RbC. We also demonstrate that phosphorylation of RbC at serines 788 and 795 destabilizes one set of RbC-E2F-DP interactions directly, while phosphorylation at threonines 821 and 826 induces an intramolecular interaction between RbC and the Rb pocket that destabilizes the remaining interactions indirectly. Our findings explain the requirement of RbC for high-affinity E2F binding and growth suppression and establish a mechanism for the regulation of Rb-E2F association by phosphorylation.

Structure of the Rb C-terminal domain bound to E2F1-DP1: a mechanism for phosphorylation-induced E2F release., Rubin SM, Gall AL, Zheng N, Pavletich NP, Cell. 2005 Dec 16;123(6):1093-106. PMID:16360038

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Disease

Known disease associated with this structure: Bladder cancer OMIM:[180200], Osteosarcoma OMIM:[180200], Pinealoma with bilateral retinoblastoma OMIM:[180200], Retinoblastoma OMIM:[180200]

About this Structure

2AZE is a 3 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Rubin SM, Gall AL, Zheng N, Pavletich NP. Structure of the Rb C-terminal domain bound to E2F1-DP1: a mechanism for phosphorylation-induced E2F release. Cell. 2005 Dec 16;123(6):1093-106. PMID:16360038 doi:10.1016/j.cell.2005.09.044

Page seeded by OCA on Tue Feb 17 13:17:28 2009

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2aze

From Proteopedia

Contents

Structure of the Rb C-terminal domain bound to an E2F1-DP1 heterodimer

Disease

About this Structure

Reference

Proteopedia Page Contributors and Editors (what is this?)

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