NATIVE ACETYLCHOLINESTERASE (E.C. 18.104.22.168) FROM TORPEDO CALIFORNICA
[[2ace]], [[Resolution|resolution]] 2.50Å
2ace is a 1 chain structure with sequence from Torpedo californica. This structure supersedes the now removed PDB entry 1ace. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
(-)-Huperzine A (HupA) is found in an extract from a club moss that has been used for centuries in Chinese folk medicine. Its action has been attributed to its ability to strongly inhibit acetylcholinesterase (AChE). The crystal structure of the complex of AChE with optically pure HupA at 2.5 A resolution shows an unexpected orientation for the inhibitor with surprisingly few strong direct interactions with protein residues to explain its high affinity. This structure is compared to the native structure of AChE devoid of any inhibitor as determined to the same resolution. An analysis of the affinities of structural analogues of HupA, correlated with their interactions with the protein, shows the importance of individual hydrophobic interactions between HupA and aromatic residues in the active-site gorge of AChE.
Structure of acetylcholinesterase complexed with the nootropic alkaloid, (-)-huperzine A.,Raves ML, Harel M, Pang YP, Silman I, Kozikowski AP, Sussman JL Nat Struct Biol. 1997 Jan;4(1):57-63. PMID:8989325
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Raves ML, Harel M, Pang YP, Silman I, Kozikowski AP, Sussman JL. Structure of acetylcholinesterase complexed with the nootropic alkaloid, (-)-huperzine A. Nat Struct Biol. 1997 Jan;4(1):57-63. PMID:8989325