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1zv6
From Proteopedia
| 1zv6, 21 NMR models () | |||||||||
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| Domains: | VHP | ||||||||
| Related: | 1qzp, 1qqv, 1vii | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
NMR structure of the human dematin headpiece S74E mutant
Dematin is an actin binding protein from the junctional complex of the erythrocyte cytoskeleton. The protein has two actin binding sites and bundles actin filaments in vitro. This actin bundling activity is reversibly regulated by phosphorylation in the carboxyl terminal "headpiece" domain (DHP). DHP is a typical villin-type headpiece actin binding motif and contains a flexible N-terminal loop and an alpha-helical C-terminal subdomain that is phosphorylated at Ser74. The NMR structure of a Ser74-to-Glu mutant (DHPs74e) closely mimics the conformation of phosphorylated DHP. The negative charge at Ser74 does not alter the conformation of the C-terminal subdomain, but attracts the N-terminal loop toward the C terminus, changing the orientation of the N-terminal subdomain. NMR relaxation studies also indicate reduced mobility in the N-terminal loop in DHPs74e. Thus, phosphorylation in DHP serves as a switch controlling the conformational state of DHP and the actin bundling activity of dematin.
A phosphorylation-induced conformation change in dematin headpiece., Jiang ZG, McKnight CJ, Structure. 2006 Feb;14(2):379-87. PMID:16472756
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1ZV6 is a 1 chain structure of sequence from Homo sapiens. Full experimental information is available from OCA.
Reference
- Jiang ZG, McKnight CJ. A phosphorylation-induced conformation change in dematin headpiece. Structure. 2006 Feb;14(2):379-87. PMID:16472756 doi:10.1016/j.str.2005.11.007
Page seeded by OCA on Tue Feb 17 11:11:28 2009
