1zr4

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1zr4, resolution 3.40Å ()
Related: 1zr2, 1gdt, 2rsl, 1gdr
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Structure of a Synaptic gamma-delta Resolvase Tetramer Covalently linked to two Cleaved DNAs

Publication Abstract from PubMed

The structure of a synaptic intermediate of the site-specific recombinase gammadelta resolvase covalently linked through Ser10 to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is substantially changed from that of a presynaptic complex between dimeric resolvase and the cleavage site DNA. Because the two cleaved DNA duplexes that are to be recombined lie on opposite sides of the core tetramer, large movements of both protein and DNA are required to achieve strand exchange. The two dimers linked to the DNAs that are to be recombined are held together by a flat interface. This may allow a 180 degrees rotation of one dimer relative to the other in order to reposition the DNA duplexes for strand exchange.

Structure of a synaptic gammadelta resolvase tetramer covalently linked to two cleaved DNAs., Li W, Kamtekar S, Xiong Y, Sarkis GJ, Grindley ND, Steitz TA, Science. 2005 Aug 19;309(5738):1210-5. Epub 2005 Jun 30. PMID:15994378

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1zr4 is a 16 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA.

See Also

Reference

  • Li W, Kamtekar S, Xiong Y, Sarkis GJ, Grindley ND, Steitz TA. Structure of a synaptic gammadelta resolvase tetramer covalently linked to two cleaved DNAs. Science. 2005 Aug 19;309(5738):1210-5. Epub 2005 Jun 30. PMID:15994378

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