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1zjm
From Proteopedia
| 1zjm, resolution 2.10Å () | |||||||||
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| Ligands: | |||||||||
| Gene: | POLB (Homo sapiens) | ||||||||
| Domains: | POLXc | ||||||||
| Related: | 1bpx | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Human DNA Polymerase beta complexed with DNA containing an A-A mismatched primer terminus
DNA polymerases occasionally insert the wrong nucleotide. For this error to become a mutation, the mispair must be extended. We report a structure of DNA polymerase beta (pol beta) with a DNA mismatch at the boundary of the polymerase active site. The structure of this complex indicates that the templating adenine of the mispair stacks with the primer terminus adenine while the templating (coding) cytosine is flipped out of the DNA helix. Soaking the crystals of the binary complex with dGTP resulted in crystals of a ternary substrate complex. In this case, the templating cytosine is observed within the DNA helix and forms Watson-Crick hydrogen bonds with the incoming dGTP. The adenine at the primer terminus has rotated into a syn-conformation to interact with the opposite adenine in a planar configuration. Yet, the 3'-hydroxyl on the primer terminus is out of position for efficient nucleotide insertion.
Nucleotide-induced DNA polymerase active site motions accommodating a mutagenic DNA intermediate., Batra VK, Beard WA, Shock DD, Pedersen LC, Wilson SH, Structure. 2005 Aug;13(8):1225-33. PMID:16084394
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1ZJM is a 4 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Batra VK, Beard WA, Shock DD, Pedersen LC, Wilson SH. Nucleotide-induced DNA polymerase active site motions accommodating a mutagenic DNA intermediate. Structure. 2005 Aug;13(8):1225-33. PMID:16084394 doi:10.1016/j.str.2005.05.010
Page seeded by OCA on Wed Feb 18 03:06:04 2009
