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1z0h
From Proteopedia
| 1z0h, resolution 2.00Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene: | botB (Clostridium botulinum) | ||||||||
| Activity: | Bontoxilysin, with EC number 3.4.24.69 | ||||||||
| Domains: | Toxin_R_bind_N, Toxin_R_bind_C | ||||||||
| Related: | 1erw | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
N-terminal helix reorients in recombinant C-fragment of Clostridium botulinum type B
Botulinum neurotoxins comprise seven distinct serotypes (A-G) produced by Clostridium botulinum. The crystal structure of the binding domain of the botulinum neurotoxin type B (BBHc) has been determined to 2A resolution. The overall structure of BBHc is well ordered and similar to that of the binding domain of the holotoxin. However, significant structural changes occur at what would be the interface of translocation and binding domains of the holotoxin. The loop 911-924 shows a maximum displacement of 14.8A at the farthest point. The N-terminal helix reorients and moves by 19.5A from its original position. BBHc is compared with the binding domain of the holotoxin of botulinum type A and B, and the tetanus C-fragment to characterize the heavy chain-carbohydrate interactions. The probable reasons for different binding affinity of botulinum and tetanus toxins are discussed.
N-terminal helix reorients in recombinant C-fragment of Clostridium botulinum type B., Jayaraman S, Eswaramoorthy S, Ahmed SA, Smith LA, Swaminathan S, Biochem Biophys Res Commun. 2005 Apr 29;330(1):97-103. PMID:15781237
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1Z0H is a 2 chains structure of sequences from Clostridium botulinum. Full crystallographic information is available from OCA.
Reference
- Jayaraman S, Eswaramoorthy S, Ahmed SA, Smith LA, Swaminathan S. N-terminal helix reorients in recombinant C-fragment of Clostridium botulinum type B. Biochem Biophys Res Commun. 2005 Apr 29;330(1):97-103. PMID:15781237 doi:10.1016/j.bbrc.2005.02.123
Page seeded by OCA on Tue Feb 17 06:38:18 2009
