First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
1yyh
From Proteopedia
| 1yyh, resolution 1.90Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene: | NOTCH1, TAN1 (Homo sapiens) | ||||||||
| Domains: | ANK | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of the human Notch 1 ankyrin domain
The Notch receptor is part of a highly conserved signalling system of central importance to animal development. Its ANK (ankyrin) domain is required for Notch-mediated signal transduction. The crystal structure of the human Notch 1 ANK domain was solved by molecular replacement at 1.9 A (1 A=0.1 nm) resolution, and it shows that the features identified in the Drosophila homologue are conserved. The domain has six of the seven ANK repeats predicted from sequence. The putative first repeat, which has only part of the consensus and a long insertion, is disordered in both molecules in the asymmetric unit, possibly due to the absence of the RAM (RBPJkappa-associated molecule) region N-terminal to it. The exposed hydrophobic core is involved in intermolecular interactions in the crystal. Evolutionary trace analysis identified several residues that map to the hairpins of the structure and may be of functional importance. Based on the Notch 1 ANK structure and analysis of homologous Notch ANK sequences, we predict two possible binding sites on the domain: one on the concave surface of repeat 2 and the other below the hairpins of repeats 6-7.
High-resolution crystal structure of the human Notch 1 ankyrin domain., Ehebauer MT, Chirgadze DY, Hayward P, Martinez Arias A, Blundell TL, Biochem J. 2005 Nov 15;392(Pt 1):13-20. PMID:16011479
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1YYH is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Ehebauer MT, Chirgadze DY, Hayward P, Martinez Arias A, Blundell TL. High-resolution crystal structure of the human Notch 1 ankyrin domain. Biochem J. 2005 Nov 15;392(Pt 1):13-20. PMID:16011479 doi:10.1042/BJ20050515
Page seeded by OCA on Tue Feb 17 05:23:33 2009
