First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
1yhn
From Proteopedia
| 1yhn, resolution 3.00Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||||
| Gene: | Rab7 (Homo sapiens), RILP (Homo sapiens) | ||||||||
| Domains: | Rab7 | ||||||||
| Related: | 1t91 | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Structure basis of RILP recruitment by Rab7
Rab7 regulates vesicle traffic from early to late endosomes, and from late endosomes to lysosomes. The crystal structure of Rab7-GTP in complex with the Rab7 binding domain of RILP reveals that Rab7 interacts with RILP specifically via two distinct areas, with the first one involving the switch and interswitch regions and the second one consisting of RabSF1 and RabSF4. Disruption of these interactions by mutations abrogates late endosomal/lysosomal targeting of Rab7 and RILP. The Rab7 binding domain of RILP forms a coiled-coil homodimer with two symmetric surfaces to interact with two separate Rab7-GTP molecules, forming a dyad configuration of Rab7-RILP(2)-Rab7. Mutations that disrupt RILP dimerization also abolish its interactions with Rab7-GTP and late endosomal/lysosomal targeting, suggesting that the dimeric form of RILP is a functional unit. Structural comparison suggests that the combined use of RabSF1 and RabSF4 with the switch regions may be a general mode of action for most Rab proteins in regulating membrane trafficking.
Structural basis for recruitment of RILP by small GTPase Rab7., Wu M, Wang T, Loh E, Hong W, Song H, EMBO J. 2005 Apr 20;24(8):1491-501. Epub 2005 Mar 31. PMID:15933719
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
Disease
Known disease associated with this structure: Epilepsy, progressive myoclonic 1B OMIM:[608500]
About this Structure
1YHN is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Wu M, Wang T, Loh E, Hong W, Song H. Structural basis for recruitment of RILP by small GTPase Rab7. EMBO J. 2005 Apr 20;24(8):1491-501. Epub 2005 Mar 31. PMID:15933719
Page seeded by OCA on Tue Feb 17 17:35:33 2009
Categories: Homo sapiens | Hong, W. | Song, H. | Wang, T. | Wu, M. | Protein transport | Rab7 | Rilp
