First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.

1xyh

From Proteopedia

Jump to: navigation, search


1xyh, resolution 2.60Å ()
Gene: PPIL3b (Homo sapiens)
Activity: Peptidylprolyl isomerase, with EC number 5.2.1.8
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Crystal Structure of Recombinant Human Cyclophilin J

Publication Abstract from PubMed

Cyclophilins (CyPs) are a large class of highly conserved ubiquitous peptidyl-prolyl cis-trans isomerases. CyPs have also been identified as being a specific receptor for the immunosuppressive drug cyclosporin A and are involved in a variety of biological functions. CyPJ is a novel member of the CyP family and human CyPJ (hCyPJ) is the protein encoded by a cyclophilin-like gene from human foetal brain, which shows 50% sequence identity to human cyclophilin A (hCyPA). Recombinant hCyPJ was expressed in Escherichia coli and purified. The three-dimensional structure of hCyPJ has been determined by molecular replacement using the hCyPA structure as the search model and has been refined at 2.6 angstroms resolution. The hCyPJ molecule contains four helices and one beta-barrel composed of eight antiparallel beta-strands. The overall secondary and tertiary structures of hCyPJ are similar to those of hCyPA, but hCyPJ contains an additional disulfide bridge and four segments with conformations that are strikingly different from those of hCyPA. His43 and Gln52 of hCyPJ are expected to be the active sites based on sequence alignment with hCyPA. The hCyPJ structure shows a conserved water molecule close to His43 and Gln52 which appears to support the solvent-assisted mechanism.

Structure of recombinant human cyclophilin J, a novel member of the cyclophilin family., Huang LL, Zhao XM, Huang CQ, Yu L, Xia ZX, Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):316-21. Epub 2005, Feb 24. PMID:15735342

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1xyh is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

See Also

Reference

  • Huang LL, Zhao XM, Huang CQ, Yu L, Xia ZX. Structure of recombinant human cyclophilin J, a novel member of the cyclophilin family. Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):316-21. Epub 2005, Feb 24. PMID:15735342 doi:10.1107/S0907444904033189

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools